Kiyoshi Hayashi

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In vitro random mutagenesis is a powerful tool for altering properties of enzymes. We describe here a novel random mutagenesis method using rolling circle amplification, named error-prone RCA. This method consists of only one DNA amplification step followed by transformation of the host strain, without treatment with any restriction enzymes or DNA ligases,(More)
Although proteins may be artificially improved by random insertion and deletion mutagenesis methods, these procedures are technically difficult, and the mutations introduced are no more variable than those introduced by the introduction of random point mutations. We describe here a three-step method called RAISE, which is based on gene shuffling and can(More)
We give in this paper some formulas which are useful in the construction of nontrivial conserved currents for submodels of CP 1-model or QP 1-model in (1 + 2) dimensions. These are full generalization of our results in the previous paper (hep-th 9802105). In the previous paper [1], we have given explicit formulas to the nontrivial conserved currents in the(More)
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