Kiyoshi Hama

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The distribution of Ca2+-binding protein, parvalbumin (PV), containing neurons and their colocalization with glutamic acid decarboxylase (GAD) were studied in the rat hippocampus and dentate gyrus using immunohistochemistry. PV immunoreactive (PV-I) perikarya were concentrated in the granule cell layer and hilus in the dentate gyrus and in the stratum(More)
Fast spiking cells in the CA1 region of the rat hippocampus were revealed as gamma-aminobutyric acid (GABA)ergic non-pyramidal cells containing the calcium-binding protein parvalbumin by intracellular injection of Lucifer yellow in vitro in combination with postembedding parvalbumin immunohistochemistry.
Aldehyde fixatives containing high concentrations of glutaraldehyde, usually used for conventional electron microscope studies, were successfully used for immunocytochemistry of transmitter synthesizing enzymes, glutamate decarboxylase and tyrosine hydroxylase, in the rat central nervous system. Although a high concentration of glutaraldehyde could cause(More)
Intracellular recording and injection of horseradish peroxidase in rat hippocampal slice preparations revealed the existence of two physiologically distinct types of non-pyramidal cells: one a fast spiking type and the other a non-fast spiking type. In the CAl pyramidal cell layer and the subgranular zone of the dentate gyrus fast spiking cells were found,(More)
Number, length, and diameters of dendritic spines of the granule cell in the dorsal leaf of the rat dentate gyrus were measured by using high-voltage electron microscope stereo images of 5-micron-thick Golgi preparations with the aid of a three-dimensional image analyzer system. Spine densities of 2.02 +/- 0.28, 2.28 +/- 0.33, and 3.36 +/- 0.35 per 1 micron(More)
The coexistence of cholecystokinin-octapeptide-like (CCK-L) and/or vasoactive-intestinal-polypeptide-like immunoreactive (VIP-LI) materials and glutamate decarboxylase (GAD) was studied in the rat hippocampus and dentate gyrus by means of immunohistochemistry. Consecutive 40-micron-thick sections were incubated in different antisera and those cells which(More)
The effects of the unilateral olfactory naris closure were studied immunohistochemically in the main olfactory bulb of adult rats. In the olfactory deprived bulbs, the tyrosine hydroxylase-immunoreactive elements in the glomeruli and the juxtaglomerular region decreased drastically, whereas the glutamate decarboxylase-immunoreactive elements in these(More)
The coexistence of immunoreactivities for glutamate decarboxylase (GAD) and tyrosine hydroxylase (TH) was revealed in some neurons in the periglomerular region and in the superficial part of the external plexiform layer of the rat main olfactory bulb. In neurons showing the immunoreactivity for either GAD or TH, about 10-55% showed both immunoreactivities.
The fine structure of the lateral line organ of the Japanese sea eel Lyncozymba nystromi has been studied with the electron microscope. The sensory epithelium of the lateral line organ consists of a cluster of two major types of cells, the sensory hair cells and the supporting cells. The sensory cell is a slender element with a flat upper surface provided(More)