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Bright and stable near infra-red fluorescent protein for in vivo imaging
TLDR
Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes.
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
TLDR
Protein retention ExM is described, a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins, for multicolor super-resolution imaging of cells and mammalian tissues on conventional microscopes.
Monomeric fluorescent timers that change color from blue to red report on cellular trafficking.
TLDR
The results indicate that LAMP-2A transport through the plasma membrane and early or recycling endosomes to lysosomes is a major pathway for LAMP -2A trafficking.
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
TLDR
A genetically encoded fluorescent voltage indicator is created, simultaneously optimizing its brightness and membrane localization using the authors' microscopy-guided cell-picking strategy, and is applicable in three model systems.
Population imaging of neural activity in awake behaving mice
TLDR
A genetically encoded fluorescent voltage indicator, SomArchon, which exhibits millisecond response times and is compatible with optogenetic control, and which increases the sensitivity, signal-to-noise ratio, and number of neurons observable several-fold over previously published fully genetically encoded reagents is described.
A genetically encoded near-infrared fluorescent calcium ion indicator
TLDR
NIR-GECO1, the first near-infrared genetically encoded calcium ion (Ca2+) indicator, enables improved Ca2+ imaging in conjunction with blue-light-activated optogenetic tools and multiplexed imaging in cell cultures and tissue slices.
Monomeric red fluorescent proteins with a large Stokes shift
TLDR
The data indicate that within 40 μm the breast cancer cells show significant polarization towards vessels in living mice, and this approach is applied to a mouse xenograft model of breast cancer to intravitally study the motility and Golgi-nucleus alignment of tumor cells as a function of their distance from blood vessels.
Extended Stokes Shift in Fluorescent Proteins: Chromophore–Protein Interactions in a Near-Infrared TagRFP675 Variant
TLDR
Based on the spectroscopic, biochemical, and structural analysis, it is suggested that the rearrangement of the hydrogen bond interactions between the chromophore and the protein matrix is responsible for the TagRFP675 spectral properties.
Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large Stokes shift.
TLDR
Crystallographic and mutagenesis analyses, as well as isotope and temperature dependences, suggest that an excited-state proton transfer (ESPT) is responsible for the LSSs observed in LSSmKates, which suggest that different chromophores formed by distinct tripeptides in different environments can be rationally modified to yield RFPs with novel photochemical properties.
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