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The widely used expression vector pBI121 for plant transformation was reconstructed, and the complete sequence of 14758 bp is now available (accession number AF485783). The T-DNA region (6193 bp) contains the right border, expression cassettes for a neomycin phosphotransferase II (NPTII) selection marker and a β-glucuronidase (GUS) reporter gene, and the(More)
A full-length cDNA (designated rcaII) encoding the Rubisco activase (RCA) of rice (Oryza sativa L.) has been cloned from a cDNA library constructed with mRNA from green leaves. Sequence analysis resulted in a reading frame of 432 amino acids with a calculated molecular mass of 47.9 kDa and an estimated isoelectric point of 5.97. The deduced amino acid(More)
Streptomycin-resistant colonies were isolated from protoplast cultures of haploid Nicotiana plumbaginifolia based on their ability to green in medium containing 1 mg/ml streptomycin sulfate. The frequency of resistant colonies was 0.9×10−5 in nonmutagenized culture, and increased ten-fold following treatment of culture with 10 μg/ml(More)
Cleome spinosa is widely used as a garden ornamental in many countries. Here we determined the optimal conditions for plant regeneration from different tissue explants grown in vitro. Induction medium containing MS salts, MS vitamins, 3% sucrose, 1 mg l−1 BA, 200 mg l−1 timentin, and 0.8% agar was sufficient for shoot regeneration of all the tissue explants(More)
Bidens pilosa L. is an erect perennial herb that is used as a folk medicine for the treatment of a variety of illnesses including diabetes. Genetic engineering is an effective means of producing desired phytocompounds in certain medicinal plants; however, plant regeneration and genetic transformation have not yet been reported for B. pilosa. Here, we(More)
In a previous study two haploid streptomycin-resistant clones of Nicotiana plumbaginifolia were isolated. The chromosome number of one of these clones has now been doubled through leaf-midvein culture and the resultant diploids were characterized genetically. Our results show that streptomycin resistance in this clone is conditioned by a recessive nuclear(More)
Phosphoenolpyruvate carboxylase (PEPC) is a key enzyme in the C4 photosynthetic pathway for the initial fixation of atmospheric CO2 into the four-carbon (C4) acid, oxaloacetate. Here, we report that a vector carrying the intact maize C4 pepc gene was used to transform C3 monocot japonica rice cultivar Tainung 67 (TNG67) which is an important cultivar in(More)
We have developed a polymerase chain reaction (PCR) method for sequencing of tobacco chloroplast genome. In a mixture containing chloroplast DNA, 5′-end-labeled oligonucleotide primer, Taq DNA polymerase and reaction buffer, we were able to sequence a segment of chloroplast 16S rRNA gene. The results showed that the 750 bp of DNA sequenced were identical to(More)