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Azorhizobium caulinodans ORS571 is able to nodulate roots and stems of the tropical legume Sesbania rostrata. An ORS571 Tn5 insertion mutant, strain ORS571-X15, had a rough colony morphology, was nonmotile, and showed clumping behavior on various media. When this pleiotropic mutant was inoculated on roots or stems of the host, no nodules developed (Nod-).(More)
The sequence surrounding the Azorhizobium caulinodans (Ac) regulatory nodD gene was analyzed. Upstream from nodD and in the opposite orientation, two small open reading frames were identified (ORF1 and ORF2). The DNA sequence corresponding to ORF1, termed epsilon 1, is similar to a part of the insertion element IS51 from Pseudomonas savastanoi. Immediately(More)
As most building energy simulation programs focus on the thermal response of the building, the relative humidity of the indoor air is often calculated in a simplified way. One of the main shortcomings is the isothermal calculation, which may have a strong influence the predicted relative humidity. In this paper the use of a simplified effective moisture(More)
As a cost-effective alternative to experiments, computational fluid dynamics (CFD) can provide new insight in airflow patterns and the related convective heat transfer (CHT). However, together with the governing equations, the description of the boundary conditions determines for a greater part the reliability and the accuracy of CFD simulations. In this(More)
A fast immunoblotting procedure, termed "microgel diffusion blotting," is used to detect and identify antibodies to extractable nuclear antigens (i.e., to Sm, RNP, and SSB) in patients with rheumatic diseases. The method differs from the standard immunoblotting techniques by the use of ultra-thin microgels for polyacrylamide gel electrophoresis: the very(More)
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