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The BRCA1 gene encodes a complex protein that appears to be involved in some aspects of DNA repair, transcription, or cell cycle regulation. The phosphorylation of BRCA1 is enhanced following episodes of DNA damage or during cell cycle progression, indicating that phosphorylation may be an important regulatory mechanism. Through a yeast two hybrid assay, we(More)
The relative concentrations of antibodies produced in monkeys against three forms of human growth hormone (hGH) were determined using an antigen-specific avidin/biotin ELISA assay. Monkeys were treated in two separate 90-day studies with recombinant methionyl-hGH (met-hGH) and pituitary-derived hGH (pit-hGH) (Study 1) and recombinant natural sequence hGH(More)
A biochemical approach was used to identify proteins which interact with human BRCA1. Through this work, a kinase activity which co-purifies with BRCA1 has been identified. This kinase activity, which phosphorylates BRCA1 in vitro, was originally identified in Sf9 insect cells but is also present in cells of human origin including breast and ovarian(More)
The protein kinase family represents an enormous opportunity for drug development. However, the current limitation in structural diversity of kinase inhibitors has complicated efforts to identify effective treatments of diseases that involve protein kinase signaling pathways. We have identified a new structural class of protein serine/threonine kinase(More)
Cyclin D1 gene overexpression is a frequent event in a number of human cancers. These observations have led to the suggestion that cyclin D1 alterations might play a role in the etiology of cancer. This possibility is supported by the finding that transfection of mammalian cells with cyclin D1 can accelerate progression through the G1 phase of the cell(More)
Age-related decreases in humoral immune function have been well documented. In aged mice, these functional deficits may be due, in part, to decreased numbers of precursor B cells. Interleukin-7 (IL-7) plays a key role in B cell development by stimulating proliferation of progenitor and pre-B cells. In the current study, proliferation of the murine(More)
A PCR-based technique was used to generate a large pool of random sequence double-stranded DNAs. Four DNA sequences that selectively bound in vitro to a mutant p53 143A protein, synthesized in baculovirus infected cells, were characterized. The four DNA sequences all approximated the known consensus sequence for wild-type p53. Wild-type p53 also bound the(More)
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