Kikuo Iwabuchi

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A continuous cell line has been established from larval fat body tissues of the cerambycid beetle Xylotrechus pyrrhoderus Bates. These cells were cultured in MGM-450 medium. The cell line, designated as XP-1, showed a heterogeneous population consisting of spherical and spindle-shaped cells with some capacity to adhere and a doubling time of 5 d. The(More)
Here we propose an environmentally robust hybrid (biotic-abiotic) robotic system that uses insect heart cells. Our group has already presented a hybrid actuator using rat heart muscle cells, but it is difficult to keep rat heart muscle cells contracting spontaneously without maintaining the culture conditions carefully. Insect cells, by contrast, are robust(More)
Living muscle tissues and cells have been attracting attention as potential actuator candidates. In particular, insect dorsal vessel tissue (DVT) seems to be well suited for a bio-actuator since it is capable of contracting autonomously and the tissue itself and its cells are more environmentally robust under culturing conditions compared with mammalian(More)
Electroantennograms were recorded from the grape borerXylotrechus pyrrhoderus in response to serial dilutions of male sex pheromone components, (2S,3S)-octanediol and (2S)-hydroxy-3-octanone, and to 100 μg of their optical isomers and host plant substances. Female antennae always responded more strongly than male antennae. Antennae of both sexes were highly(More)
We have established a continuous cell line from the fat body tissue of the longicorn beetle Plagionotus christophi. The cells have been serially subcultured in MGM450 medium, and the line has been designated as PC-1. The cells were grown in suspension and comprise largely flattened spindle- or oval-shaped cells morphologically related to blood cells of(More)
Endoparasitoids have the ability to evade the cellular immune responses of a host and to create an environment suitable for survival of their progeny within a host. Generally, the host immune system is suppressed by endoparasitoids. However, polyembryonic endoparasitoids appear to invade their hosts using molecular mimicry rather than immune system(More)
Observations made in an outdoor cage showed that most pairings of the grape borerXylotrechus pyrrhoderus Bates were formed via inflight orientation of females towards pheromone-releasing males. The male sex pheromone induced orientation behavior in flying females, as well as walking approaches and prolonged lingering periods near the pheromone source in(More)
The male sex pheromone of the longicorn beetle, Xylotrechus pyrrhoderus pyrrhoderus Bates (Cerambycidae: Tribe Clytini) plays an important role in attracting females. This pheromone is produced by the pheromone gland located in the prothorax. However, the detailed structure and underlying developmental process of this gland are still unknown. We(More)
The number of insect midgut cells is maintained homeostatically in vivo and in vitro. However, during starvation, the midgut shrinks and the rate of cell replacement appears to be suppressed. When they undergo metamorphosis, the internal organs of insects are drastically remodeled by cell proliferation, differentiation, and apoptotic processes, and the net(More)
  • K. Iwabuchi
  • In Vitro Cellular & Developmental Biology…
  • 1995
Single two-cell-stage embryos of a polyembryonic waspCopidosoma floridanum cultured in 20 µl droplets of culture medium developed to morulae at the same developmental rate as those in host eggs, but the subsequent development into polymorulae was inferior. This inferior development was markedly improved by addition of juvenile hormone (I, II, or III) or its(More)