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The population structure of Mycobacterium tuberculosis is typically clonal therefore genotypic lineages can be unequivocally identified by characteristic markers such as mutations or genomic deletions. In addition, drug resistance is mainly mediated by mutations. These issues make multiplexed detection of selected mutations potentially a very powerful tool(More)
The human immunodeficiency virus type 1 envelope glycoprotein (Env) complex is the principal focus of neutralizing antibody-based vaccines. The functional Env complex is a trimer consisting of six individual subunits: three gp120 molecules and three gp41 molecules. The individual subunits have proven unsuccessful as vaccines presumably because they do not(More)
Salmonella enterica serovar Typhi, the causative agent of typhoid fever, is highly clonal and genetically conserved, making isolate subtyping difficult. We describe a standardized multiplex ligation-dependent probe amplification (MLPA) genotyping scheme targeting 11 key phylogenetic markers of the S. Typhi genome. The MLPA method demonstrated 90%(More)
OBJECTIVE To assess whether exon deletions or duplications in CLCN1 are associated with recessive myotonia congenita (MC). METHODS We performed detailed clinical and electrophysiologic characterization in 60 patients with phenotypes consistent with MC. DNA sequencing of CLCN1 followed by multiplex ligation-dependent probe amplification to screen for exon(More)
Multiplex ligation-dependent probe amplification (MLPA) is a powerful tool to identify genomic polymorphisms. We have previously developed a single nucleotide polymorphism (SNP) and large sequence polymorphisms (LSP)-based MLPA assay using a read out on a liquid bead array to screen for 47 genetic markers in the Mycobacterium tuberculosis genome. In our(More)
Both culture and serological methods are used to diagnose enteric fever. An anti-Salmonella lipopolysaccharide O12 monoclonal antibody could be used to detect Salmonella organism directly from routine blood culture broths. Blood from 78 young outpatients suspected of having enteric fever was incubated in enrichment broth, and after 2 or 4 days, broth(More)
We have generated a panel of murine monoclonal antibodies (mAbs) directed against recombinant human interleukin-2 (rh IL-2). All mAbs have similar affinities (Kaff approximately equal to 10(-8) M) and are of the IgG1 isotype. Specificity of the mAbs has been established using an ELISA method and immunoprecipitation of native human interleukin-2 (nh IL-2)(More)
Identification of Salmonella enterica serovar Typhi genotypes using rapid Multiplex Ligation1 dependent Probe Amplification (MLPA) 2 3 Duy Pham Thanh , Nga Tran Vu Thieu , Chau Tran Thuy , Martin Lodén , Kiki Tuin , 4 James I Campbell 1, , Nguyen Van Minh Hoang , Phat Voong Vinh , Jeremy J Farrar 1, , Kathryn E Holt , 5 Gordon Dougan , and Stephen Baker 1,(More)
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