Ki - Bum Lee

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Protein phosphorylation, while critical to cellular behavior, has been undercharacterized in pluripotent cells. Therefore, we performed phosphoproteomic analyses of human embryonic stem cells (hESCs) and their differentiated derivatives. A total of 2546 phosphorylation sites were identified on 1602 phosphoproteins; 389 proteins contained more(More)
Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes.(More)
A 1-year-old girl presented with a 10-month history of progressive protuberance of the left frontal skull. Magnetic resonance imaging and computed tomography demonstrated a large osteolytic interosseous mass extending to the frontal sinus and temporal base without any intracranial invasion. A fronto-temporo-parietal craniectomy of the outer skull table and(More)
Herein, we report the use of a graphene-based nanomaterial for the design of hybrid nanofi brous scaffolds to guide NSC differentiation into oligodendrocytes ( Figure 1 ). Graphene-based nanomaterials, such as graphene oxide (GO), have recently gained considerable interest for tissue engineering applications due to their favorable chemical, electrical and(More)
Hybrid Materials for Bioapplications Perry T. Yin,† Shreyas Shah,‡ Manish Chhowalla, and Ki-Bum Lee*,†,‡,∥ †Department of Biomedical Engineering, ‡Department of Chemistry and Chemical Biology, Department of Materials Science and Engineering, and Institute for Advanced Materials, Devices, and Nanotechnology (IAMDN), Rutgers, The State University of New(More)
Nanomaterials such as silicon nanowires (SiNWs), [ 1 , 2 ] carbon nanotubes (CNTs), [ 3–6 ] and graphene, [ 7,8 ] have gained much attention for use in electrical biosensors due to their nanoscopic and electrical properties. For instance, SiNWs and CNTs can be integrated into fi eld-effect transistors (FETs) to detect small amounts of target biomolecules(More)
Achieving a controlled and reproducible means to direct stem cell differentiation is the single most critical concern scientists have been trying to address since the discovery of stem cells. In this regard, the use of small molecules and RNA interference offers unique advantages by targeting different cellular mechanisms. Our cyclodextrin-modified(More)
We describe the development of a versatile fluorescence resonance energy transfer (FRET)-based real-time monitoring system, consisting of (a) coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) as the drug carrier, (b) a fluorescein isothiocyanate-β-cyclodextrin (FITC-β-CD) as redox-responsive molecular valve blocking the pores, and(More)
BACKGROUND/AIMS Oxidative stress results in protein oxidation and is implicated in carcinogenesis. Sulfiredoxin (Srx) is responsible for the enzymatic reversal of inactivated peroxiredoxin (Prx). Nuclear factor E2-related factor 2 (Nrf2) binds to antioxidant responsive elements and upregulates the expression of Srx and Prx during oxidative stress. We aimed(More)
Stimuli-responsive drug delivery vehicles have garnered immense interest in recent years due to unparalleled progress made in material science and nanomedicine. However, the development of stimuli-responsive devices with integrated real-time monitoring capabilities is still in its nascent stage because of the limitations of imaging modalities. In this(More)