Kevin Wehner

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HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC(More)
This paper presents a generalized diversion control model for freeway incident management that is capable of concurrently optimizing the detour rates and arterial signal timings over multiple roadway corridor segments between the freeway and its neighboring arterial. To capture various operational complexities due to the interactions between multiple(More)
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