Kevin S. Lorenz

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Digital image analysis is a fundamental component of quantitative microscopy. However, intravital microscopy presents many challenges for digital image analysis. In general, microscopy volumes are inherently anisotropic, suffer from decreasing contrast with tissue depth, lack object edge detail and characteristically have low signal levels. Intravital(More)
Intravital microscopy has been recognized for its ability to make physiological measurements at cellular and subcellular levels while maintaining the complex natural microenvironment. Two-photon microscopy (TPM), using longer wavelengths than single-photon excitation, has extended intravital imaging deeper into tissues, with minimal phototoxicity. However,(More)
Optical microscopy exhibits many challenges for digital image analysis. In general, microscopy volumes are inherently anisotropic, suffer from decreasing contrast with tissue depth, and characteristically have low signal levels. This paper describes a method to segment multiphoton fluorescent microscopy images via a combination of segmentation and(More)
Intravital microscopy is a uniquely powerful tool, providing the ability to characterize cell and organ physiology in the natural context of the intact, living animal. With the recent development of high-resolution microscopy techniques such as confocal and multiphoton microscopy, intravital microscopy can now characterize structures at subcellular(More)
Three dimensional image volumes collected using optical microscopy exhibit many characteristics that cause difficulty in segmentation. These include decreasing image contrast with increasing tissue depth, poor edge detail with regard to cellular structures, and limited spatial resolution. This paper describes a three dimensional segmentation method(More)
This paper considers the formulation of a digital pre-distorter to eliminate distortions that are generated by a frequency-dependent imbalance between the analog in-phase (I) and quadrature (Q) channels of a direct conversion transmitter. Estimation of the imbalance occurs in the frequency domain, while compensation occurs in the time domain. We demonstrate(More)
ii Dedicated to my parents and my sisters. iii ACKNOWLEDGMENTS This is a great opportunity for me to share my tears of joy with loved ones and to express my immense gratitude to those who helped me on my academic journey. The memory of my first day at Purdue on June 6, 2002 is still very vivid for me. It is hard for me to believe that this long journey has(More)
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