Kevin Mcglone

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The enzyme myristoyl-CoA:protein N-myristoyltransferase is responsible for the attachment of a myristoyl group to the N-terminal glycine of a number of cell, viral and fungal proteins. In order to overcome the difficulties of purification of this enzyme from tissue sources, we have produced an N-terminally polyhistidine-tagged version of the enzyme and(More)
A protein of 80 kDa apparent molecular mass was found to be specifically myristolylated in rat brain cytosols derived from either whole brain or synaptosomes. The attachment of the fatty acid took place in the absence of protein synthesis, since the cytosols did not incorporate [14C]lysine into protein, nor did cycloheximide affect the incorporation of the(More)
This study tested hypotheses about the consistency of specified outcomes with strength of program treatment indexed by time spent in a given activity in the Systemic Lupus Erythematosus Self-Help (SLESH) course. Participants had significant increases in enabling skills and in use of relaxation and exercise activities. Participants also had significantly(More)
Antisera have been raised to three synthetic peptides based on the sequence of human myristoyl-CoA:protein N-myristoyl transferase (NMT) and to the purified enzyme following its expression in Escherichia coli. These antisera have been affinity purified and shown to react both with the E. coli expressed human NMT, and specifically with a protein of molecular(More)
The enzyme myristoyl-CoA:protein N-myristoyltransferase (NMT; EC catalyses the transfer of myristic acid to the N-terminal glycine residue of cell and viral proteins. In this report the purification and partial sequencing of this enzyme from bovine brain is described. Using a combination of ammonium sulphate precipitation, chromatography on(More)
  • C O M P E T E N T, C O N F I D E N T, +21 authors Jinjin Yang
  • 2012
National Standards (APENS) Web site documents the evolution of adapted physical education as a movement that has steadily gained traction in providing opportunities for disabled individuals to access physical education programs and curricula: The past century has seen a revolution in the way society views people with disabilities and in the way that people(More)
An enzyme activity in rat brain, capable of catalysing the transfer of myristic acid from myristoyl CoA to the amino terminus of synthetic peptides, has been characterised. The synthetic peptides used as substrates were one based on the N-terminal eight amino acids of cyclic AMP-dependent protein kinase and another hexadecapeptide based on the N-terminal(More)