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Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p
It is reported that pure, active HOPS complex binds phosphoinositides and the PX domain of the vacuolar SNARE protein Vam7p, which may be a key factor for coupling Rab GTPase activation to SNARE complex assembly. Expand
Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion
SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole fusion are combined in the six‐subunitExpand
Hierarchy of protein assembly at the vertex ring domain for yeast vacuole docking and fusion
The findings provide a unique view of the functional relationships between GTPases, SNAREs, and actin in membrane fusion, and the HOPS complex, at the end of the vertex assembly hierarchy, is most enriched at those vertices with abundant Ypt7p, which is at the start of the hierarchy. Expand
trans-SNARE complex assembly and yeast vacuole membrane fusion
It is suggested that efficient fusion requires trans-SNARE complex associations with factors such as HOPS and subsequent regulated lipid rearrangements, and that inhibitors that target Ypt7p, HOPS, or key regulatory lipids prevent trans- SNARE complex assembly and ensuing fusion. Expand
A soluble SNARE drives rapid docking, bypassing ATP and Sec17/18p for vacuole fusion
It is reported that recombinant Vam7p (rVam 7p) enters into complex with other SNAREs in vitro and bypasses the need for Sec17p, Sec18p, and ATP, which may explain why nyv1Δ strains lack the vacuole fragmentation seen with mutants in other fusion catalysts. Expand
Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition
Optically recorded circuit activity in behaving animals while manipulating circuit function using mutations, optogenetics, and drugs identifies discrete signals that entrain or detach the circuit from the locomotion central pattern generator to produce active and inactive states. Expand
LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans
It is found that the Notch-Delta pathway was required to distinguish target from non-target muscles, and the LIN-12/Notch signaling specifies target selection by selectively up-regulating guidance molecules and forming muscle arms in target cells. Expand
Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying
The results show that the HSN neurons use serotonin and the neuropeptide NLP-3 as partially redundant cotransmitters that together stimulate and coordinate activity of the target cells onto which they are released. Expand
Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans
It is found that ERG-null mutants lay eggs too frequently, and that ERg function is necessary and sufficient in the egg-laying muscles to limit egg laying, and ERG channels set postsynaptic excitability at a threshold so that further adjustments of excitability by serotonin generate two distinct behavioral states. Expand
Local neuropeptide signaling modulates serotonergic transmission to shape the temporal organization of C. elegans egg-laying behavior
By linking the in vivo actions of specific neuropeptide signaling systems with the generation of stable behavioral outcomes, this study reveals how cycles of neuromodulation emanating from non-neuronal cells can fundamentally shape the organization of a behavioral program. Expand