Kevin Hackett

Learn More
WHEN E. O. WILSON PROCLAIMED THAT INSECTS ARE THE “little creatures who run the world” (1), he was simply reaffi rming the long-recognized dominance of the largest class of animals on our planet. Insects constitute approximately 53% of all living species, with one group alone (the ants), accounting for almost a quarter of terrestrial animal biomass (2).(More)
Progenies from some wild-caught females of Drosophila willistoni and three other sibling species are entirely female. The proclivity for production of unisexual female progeny by these flies was named the sex ratio (SR) trait and was originally thought to be genetic. However, experiments in the laboratory of Donald F. Poulson in the early 1960s demonstrated(More)
Twenty-one triply cloned spiroplasma strains from the United States east of the Rocky Mountains, all isolated from tabanid (Diptera:Tabanidae) flies or serologically related to strains from tabanids, were compared reciprocally by spiroplasma deformation (DF) and metabolism inhibition (MI) serological tests. Many of the strains were also tested against 28(More)
Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R(2)). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms(More)
Significant changes have been made in the systematics of the genus Spiroplasma (class Mollicutes) since it was expanded by revision in 1987 to include 23 groups and eight sub-groups. Since that time, two additional spiroplasmas have been assigned group numbers and species names. More recently, specific epithets have been assigned to nine previously(More)
Spiroplasmas were observed in seven species of the family Tabanidae (horse flies and deer flies). This is the fifth family of the order Diptera now known to harbor spiroplasmas. Noncultivable spiroplasmas were seen in the hemolymph of three species of the genus Tabanus, and cultivable forms were isolated from the guts of six species in three genera.(More)
A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro.(More)
An unidentified Babesia was seen in a blood smear from a cat showing signs of anaemia. The cat responded to treatment with diminazene (Berenil). The morphology of the parasite is described and a comparison is made with other Babesia which have been described from the domestic cat and wild felids. This parasite most closely resembled B. herpailuri described(More)
Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin, Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight(More)
Further analysis of three sterol-nonrequiring Mollicutes (strains PS-1, TAC, and YJS) isolated from gut fluids of insects confirms their similarity to Acholeplasma. They are serologically distinct from acholeplasmas of vertebrates and several other sterol nonrequiring Mollicutes isolated from plant surfaces. The PS-1 strain had a DNA G + C content of 31 mol(More)