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Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R(2)). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms(More)
Significant changes have been made in the systematics of the genus Spiroplasma (class Mollicutes) since it was expanded by revision in 1987 to include 23 groups and eight sub-groups. Since that time, two additional spiroplasmas have been assigned group numbers and species names. More recently, specific epithets have been assigned to nine previously(More)
Several spiroplasmas (helical, motile mollicutes) were previously shown to contain extrachromosomal DNA (E-DNA) elements in the form of viruses (double-stranded viruses or the replicative form of single-stranded viruses) or plasmids. These elements are now being investigated as potential vectors for use in spiroplasma transformation systems. Described(More)
Characterization of an extrachromosomal element from an organism in the genus Spiroplasma is likely to be an essential step in the development of cloning vectors which replicate in these organisms. A restriction map for an 11-kb element, designated pCT-1, isolated from Spiroplasma taiwanese strain CT-1 (ATCC 43302) has been constructed using the restriction(More)
Progenies from some wild-caught females of Drosophila willistoni and three other sibling species are entirely female. The proclivity for production of unisexual female progeny by these flies was named the sex ratio (SR) trait and was originally thought to be genetic. However, experiments in the laboratory of Donald F. Poulson in the early 1960s demonstrated(More)
An unidentified Babesia was seen in a blood smear from a cat showing signs of anaemia. The cat responded to treatment with diminazene (Berenil). The morphology of the parasite is described and a comparison is made with other Babesia which have been described from the domestic cat and wild felids. This parasite most closely resembled B. herpailuri described(More)
Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). This publication reports research involving pesticides. It does not contain recommendations for their use, nor does it imply that uses(More)
We tested the ability of 62 growing strains belonging to the class Mollicutes to reduce the redox indicator and free-radical generator 1,1'-dibenzyl-4,4'-bipyridinium dichloride (benzyl viologen [BV]) to a blue-violet-purple color. BV was reduced by 12 Acholeplasma species but not by Acholeplasma multiforme PN525T (T = type strain). BV was also reduced by(More)
Maternal Addison's disease (primary hypoadrenalism) is an infrequent complication of pregnancy that poses a high risk of maternal mortality if not recognized and treated. If adequate steroid replacement therapy is provided, however, there is little risk for the mother during pregnancy. We treated a woman for previously undiagnosed Addison's disease during(More)