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Investigating the function of individual synapses is essential to understanding the mechanisms that influence the efficacy of chemical synaptic transmission. The known simplicity of the synaptic structure at the crayfish neuromuscular junction (NMJ) and its quantal nature of release allows an assessment of discrete synapses within the motor nerve terminals.(More)
The effects of para-chlorophenylalanine (PCPA) and 3,4 methylenedioxy-methamphetamine (MDMA, 'ecstasy') were investigated in relation to development, behavior and physiology in larval Drosophila. PCPA blocks the synthesis of serotonin (5-HT) and MDMA is known to deplete 5-HT in mammalian neurons; thus these studies were conducted primarily to target the(More)
With the simplicity of the synaptic structure and physiology at neuromuscular junctions (NMJs) of crayfish and the given transmitter being released in quantal packets, a detailed assessment in the fundamental processes of chemical synaptic transmission is possible. Since the quantal event is the basic element of transmission, we consider an approach to(More)
Acknowledgments: We are very thankful to Dr. Josef Dudel (Physiologishes Institute der Ludwig-Maximillians-Universität München) for his suggestions during this study. ABSTRACT The reserve pool (RP) and readily releasable pool (RRP) of synaptic vesicles within presynaptic nerve terminals were physiologically differentiated into distinctly separate functional(More)
The reserve pool (RP) and readily releasable pool (RRP) of synaptic vesicles within presynaptic nerve terminals were physiologically differentiated into distinctly separate functional groups. This was accomplished in glutamatergic nerve terminals by blocking the glutamate transporter with dl-threo-beta-benzyloxyaspartate (TBOA; 10 microM) during electrical(More)
Studies investigating the relationship between the use of inflectional morphology and speech-perception abilities in children with SLI traditionally have employed synthetic speech stimuli. The purpose of this study was to replicate the findings reported in Leonard, McGregor, and Allen (1992) with an older group of children with SLI and to determine if the(More)
We provide both theoretical and computational improvements to the analysis of synaptic transmission data. Theoretically, we demonstrate the correlation structure of observations within evoked postsynaptic potentials (EPSP) are consistent with multiple random draws from a common autoregressive moving-average (ARMA) process of order (2, 2). We use this(More)
  • M Desai-Shah, K Viele, G Sparks, J Nadolski, B Hayden, V K Srinivasan +1 other
  • 2008
An enhanced buildup of [Ca 2+ ] i occurs during short-term facilitation (STF) at the crayfish neuromuscular junction (NMJ). As a model system, this NMJ allows discrete postsynaptic quantal events to be counted and characterized in relation to STF. Providing 10 pulses, at 20 and 40Hz, we monitored postsynaptic quantal events over a discrete region of a nerve(More)
The release of transmitter at neuromuscular junctions (NMJ) of the opener muscle in crayfish is quantal in nature. This NMJ offers the advantage of being able to record quantal events at specific visually identified release sites, thus allowing measurement of the physiological parameters of vesicle release and its response to be directly correlated with(More)