Keqiang Shao

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We analyzed the composition and diversity of a bacterial community to determine its response to increasing salinity in the Xiangsi Lake wetland in the arid region of northwest China. We studied 12 sites, ranging from freshwater to saltwater habitats, using polymerase chain reaction (PCR)-based terminal restriction fragment length polymorphism (T-RFLP) and(More)
Bacterial community structure and the effects of several environmental factors on bacterial community distribution were investigated in the sediment of the macrophyte-dominated and algae-dominated areas in a large, shallow, eutrophic freshwater lake (Lake Taihu, China). Surface sediment samples were collected at 6 sampling sites (3 sites from each of the 2(More)
Vertical diversity of sediment bacterial communities in 2 different trophic states (macrophyte-dominated and algae-dominated) of the large shallow eutrophic Lake Taihu, China, were investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA sequence analysis. Clustering analysis of DGGE profiles showed that different clusters were(More)
A mesocosm experiment was used to study the response of a freshwater bacterial community to increasing salinity. Bacterial community composition in the control and saline groups was analyzed using polymerase chain reaction (PCR)-terminal restriction fragment length polymorphism (T-RFLP) of the 16S rRNA genes, followed by clonal sequencing of eight selected(More)
Natural and anthropogenic salinization continuously impacts inland aquatic ecosystems. Associated bacterial biofilms respond rapidly to environmental conditions and are potential bioindicators for changes in water quality. This study evaluates the effects of different salinity concentrations (0.3‰-10‰) on bacterial biofilms communities grown in fresh water(More)
Salinity was found to be the dominating contributor controlling bacterial community composition (BCC) and the abundance of Betaproteobacteria in the oligosaline Lake Bosten. The high percentage of unclassified bacteria inhabiting this unique habitat highlights the potential ecological importance of BCC in the early stage of lake salinization and(More)
The changes of taxa within the particle-attached bacterial assemblage during the decomposition of Microcystis blooms were investigated under darkness and anoxic condition in mesocosm experiments. During 14 days of darkness incubation, chlorophyll-a (Ch-a) concentration decreased from 2000 μg/L to 5 μg/L. Samples were collected on days 0, 2 and 14 for(More)
The effect of decomposition of Microcystis blooms on the sediment bacterial communities was investigated in a 14-day mesocosm experiments under dark and anoxic condition with three differing levels of Microcystis spp. biomass (from 20 to 2000 µg L(-1)) as measured by chlorophyll-a (Chl-a) concentration in the water column. Sediment samples were collected on(More)
To elucidate the relationship between particle-attached (PA, ≥ 5.0 μm) and free-living (FL, 0.2-5.0 μm) bacterial communities, samplings were collected seasonally from November 2011 to August 2012 in Meiliang Bay, Lake Taihu, China. We used 454 pyrosequencing of 16S rRNA genes to study bacterial diversity and structure of PA and FL communities. The analysis(More)
Limnetic habitats that are dominated by either algae or macrophytes represent the 2 dominant ecosystems in shallow lakes. We assessed seasonal variations in the diversity and abundance of alkaline phosphate-encoding genes (phoX) in these 2 zones of Lake Taihu, which is a large, shallow, eutrophic lake in China. There was no significant difference in(More)