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Biosynthesis, Purification, and Substrate Specificity of Severe Acute Respiratory Syndrome Coronavirus 3C-like Proteinase*
The 3C-like proteinase of severe acute respiratory syndrome (SARS) coronavirus has been proposed to be a key target for structural-based drug design against SARS. In order to understand the activeExpand
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“Pseudo” γ-Butyrolactone Receptors Respond to Antibiotic Signals to Coordinate Antibiotic Biosynthesis*
  • G. Xu, J. Wang, +5 authors H. Tan
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 18 June 2010
In actinomycetes, the onset of secondary metabolite biosynthesis is often triggered by the quorum-sensing signal γ-butyrolactones (GBLs) via specific binding to their cognate receptors. However, theExpand
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Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator
  • L. Wang, X. Tian, +5 authors H. Tan
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 26 May 2009
In bacteria, many “atypical” response regulators (ARRs) lack the conserved residues important for phosphorylation by which typical response regulators switch their output response, suggesting theExpand
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Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein
Abstract Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in theExpand
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3C-like proteinase from SARS coronavirus catalyzes substrate hydrolysis by a general base mechanism.
SARS 3C-like proteinase has been proposed to be a key enzyme for drug design against SARS. Lack of a suitable assay has been a major hindrance for enzyme kinetic studies and a large-scale inhibitorExpand
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The substrate specificity of SARS coronavirus 3C-like proteinase
Abstract The 3C-like proteinase of severe acute respiratory syndrome coronavirus (SARS) has been proposed to be a key target for structural based drug design against SARS. We have designed andExpand
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A novel role of ‘pseudo’γ‐butyrolactone receptors in controlling γ‐butyrolactone biosynthesis in Streptomyces
In streptomycetes, a quorum‐sensing mechanism mediated by γ‐butyrolactones (GBLs) and their cognate receptors was known to trigger secondary metabolism and morphological differentiation. However,Expand
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A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum
BackgroundClostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification andExpand
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Angucyclines as signals modulate the behaviors of Streptomyces coelicolor
  • W. Wang, Junjie Ji, +5 authors K. Yang
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 28 March 2014
Significance This work addresses the molecular basis for interspecies signaling effects of antibiotics, which have been a controversial but potentially significant emerging topic over the last fewExpand
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Chlorobenzylidine-calf thymus DNA interaction II: circular dichroism and nuclear magnetic resonance studies.
The binding of chlorobenzylidine to calf thymus DNA has been studied in detail by means of circular dichroism (CD), nuclear magnetic resonance (1H NMR), viscosimetry and denaturation temperatureExpand
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