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Yeast-based in vivo cloning is useful for cloning DNA fragments into plasmid vectors and is based on the ability of yeast to recombine the DNA fragments by homologous recombination. Although this method is efficient, it produces some by-products. We have developed an "ultra-low background DNA cloning system" on the basis of yeast-based in vivo cloning, by(More)
To develop a robot that behaves flexibly in the real world, it is essential that it learns various necessary functions autonomously without receiving significant information from a human in advance. Among such functions, this paper focuses on learning “prediction” that is attracting attention recently from the viewpoint of autonomous learning. The authors(More)
Acenes can be thought of as one-dimensional strips of graphene and they have the potential to be used in the next generation of electronic devices. However, because acenes larger than pentacene have been found to be unstable, it was generally accepted that they would not be particularly useful materials under normal conditions. Here, we show that, by using(More)
The purpose of the present study was to relate 3D acceleration patterns of the lower and upper trunk during running to running gait cycle, assess the validity of stride duration estimated from acceleration patterns, investigate speed-dependent changes in acceleration, and examine the test-retest reliability of these parameters. Thirteen healthy young men(More)
The authors have propounded that various functions emerge purposively and harmoniously through reinforcement learning with a neural network. In this paper, emergence of deterministic ”exploration” behavior, which is different from the stochastic exploration and needs higher intelligence, is focused on. In order to realize the intelligent exploration(More)
In this paper, the authors first point the importance of three factors for filling the gap between humans and robots in the flexibility in the real world. Those are (1)parallel processing, (2)emergence through learning and solving “what” problems, and (3)abstraction and generalization on the abstract space. To explore the possibility of(More)
The title compound, C40H46N2 {systematic name: 12,30-di-aza-hepta-cyclo[21.13.1.1(5,19).1(6,18).1(10,14).1(24,36).1(28,32)]do-tetra-conta-1(37),5(40),6(41),10(42),11,13,18,23,28,30,32(39),36(38)-dodeca-ene}, has syn-anti-syn geometry wherein the two outer [3.3]meta-cyclo-phane (MCP) moieties have a syn geometry, and contain the facing benzene and pyridine(More)
The synthesis, structure, optical and redox properties, and electronic structure of tetrakis(pentafluorophenyl)tetrathiaisophlorin dioxide (12) are reported. Oxidation of tetrakis(pentafluorophenyl)tetrathiaisophlorin (11) with dimethyldioxirane afforded the oxidized product, which was the tetrathiaisophlorin with two thiophene 1-oxide moieties (12). More(More)
In the title linear homo-trinuclear complex, [Cd3(C19H18Cl2N2O2)2(C2H3O2)2], the central Cd(II) atom is located on a centre of inversion and has a distorted octa-hedral coordination geometry formed by four O atoms from two bidentate/tetra-dentate Schiff base ligands and two O atoms from two bridging acetate ligands. The coordination geometry of the terminal(More)