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Single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly
TLDR
This experiment is the first demonstration of enzymatic formation of a ribosomal subunit in vitro from its precursor via the action of an assembly factor and it is demonstrated that the 45S particle that accumulates in ΔrlmE is a genuine precursor that can be assembled into the 50S subunit. Expand
Biogenesis and iron-dependency of ribosomal RNA hydroxylation
TLDR
A novel biosynthetic pathway for RNA hydroxylation and its response to iron is revealed, with rlhA belonging to a family of enzymes with an uncharacterized peptidase U32 motif and conserved Cys residues in the C-terminal region suggesting that this protein is directly involved in formation of ho5C2501. Expand
The ribosomal A-site finger is crucial for binding and activation of the stringent factor RelA
TLDR
Through binding assays and enzymology, it is shown that E. coli RelA does not form a stable complex with, and is not activated by, deacylated tRNA off the ribosome, which indicates that in the cell, RelA first binds the empty A-site and then recruits tRNA rather than first binding tRNA and then binding the Ribosome. Expand
Biogenesis and functions of aminocarboxypropyluridine in tRNA
TLDR
It is found that the Escherichia coli yfiP gene, which is renamed tapT (tRNA aminocarboxypropyltransferase), is responsible for acp3U formation in tRNA, a highly conserved modification found in variable- and D-loops of tRNAs. Expand
Depletion of S-adenosylmethionine impacts on ribosome biogenesis through hypomodification of a single rRNA methylation
TLDR
Analysis of the methylation status of ribosomal RNAs and transfer RNAs in Escherichia coli Δmtn strain in which cellular SAM was down-regulated found hypomodification of several methylation sites, including 2′-O-methylation at position 2552 (Um2552) of 23S rRNA. Expand
Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes.
We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N6-methyladenosineExpand