Kenneth W. Brunson

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A syngeneic tumor model system with the RAW117 lymphosarcoma cell line was developed for use in investigations of host and tumor cell properties associated with an enhanced state of malignancy. This BALB/c mouse model was found to be similar to human lymphosarcoma in that liver and spleen were the major organs involved. Sequential in vivo selections were(More)
The cytotoxicity of cationic peptides MCP-1 and MCP-2 isolated from rabbit alveolar macrophages was tested against two tumor cell lines of murine lymphosarcoma origin, RAW117-P and RAW117-H10, and a normal mouse connective tissue fibroblast strain, ATCC CCL1.RAW117-H10 is a highly malignant metastatic variant derived from the less malignant RAW117-P. Our(More)
A major goal of treatment strategies for cancer is the development of agents which can block primary tumor growth and development as well as the progression of tumor metastasis without any treatment associated side effects. Using mini peptide display (MPD) technology, we generated peptides that can bind to the human vascular endothelial growth factor (VEGF)(More)
Earlier reports from our laboratory showed that Abelson virus-induced, highly malignant and liver metastatic RAW117-H10 cells, but not the parental, less metastatic RAW117-P cells, inhibited both T-cell and B-cell mitogen-induced proliferation of syngeneic normal murine spleen cells. Similar inhibition was also noted when RAW117-H10 cell surface molecules(More)
Tumor metastasis is a multistep process which is dependent on both host and tumor properties. It is proposed that the interaction of normal host blood and endothelial cells with circulating malignant cells result in tumor cell arrest leading to subsequent metastases at specific secondary sites. In experimental animal models the frequency and location of(More)
The Abelson-virus-induced murine lymphosarcoma cell line RAW117-P and its in vivo selected highly malignant and metastatic variant RAW117-H10 have been studied for their growth in vivo, in vitro and in semi-solid agar. The highly malignant metastatic variant RAW117-H10 cells killed syngeneic Balb/c mice rapidly and formed 100-200 times more gross liver(More)
The highly malignant and metastatic RAW117-H10 cell line was developed by in vivo selection from the Abelson leukemia virus induced parental RAW117-P lymphoma. In this study we have characterized these cell lines with regard to their expression of lymphocyte and macrophage differentiation antigens, adherence, phagocytic properties, binding of various(More)
Four continuous cell lines constructed by transfecting BALB/c mouse fibroblast cells with an expression system that has the mutant c-Ha-ras gene under control of a truncated version of the mouse metallothionein-1 (mt-1) promoter were characterized for zinc-induced phenotype switching. These cells were selected for transformation in the presence of zinc, a(More)
A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces.(More)