Kenneth R. Barker

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Numerous procedures have been used to stain and clear nematode-infected plant tissues (7). Staining with acid fuchsin and destaining with lactophenol (4,7) has been the most widely used technique. More rap id means, of clearing and staining roots include autoclaving (1), exposure to saturated chloral hydrate (6), and root macera t ion after staining (3).(More)
A series of greenhouse, phytotron, field, and microplot experiments evaluated factors that influenced plant-growth.stimulation associated with the use of the pesticide aldicarb. A phytotron experiment showed.that aldicarb increased growth, of Ransom soybean at all temperatures but was somewhat phytotoxic to Coker 156 soybean at 30 C. Soybean gave the(More)
Two efficient, semi-automatic elutriators for assaying soil samples for nematodes are described. The first apparatus is a four-unit elutriator which combines conventional extraction methods with the following major features: automatic mixing of 500- to 1,500-cm(3) soil samples with water (+/- air); "turbinate" sample splitters from which fractions of 1/15(More)
Early researchers identified key concepts and developed tactics for multiple-option management of nematodes. Although the emphasis on integrated pest management over the past three decades has promoted strategies and tactics for nematode management, comprehensive studies on the related soil biology-ecology are relatively recent. Traditional management(More)
Microplot experiments were conducted to evaluate the effects of inoculum level and time of application of Paecilomyces lilacinus on the protection of tomato against MeIoidogyne incognita. The best protection against M. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase(More)
The potential of 13 Paecilomyces lilacinus isolates from various geographic regions as biocontrol agents against Meloidogyne incognita, the effects of temperature on their growth, and the characterization of the impact of soil temperature on their efficacy for controlling this nematode were investigated. Maximum fungal growth, as determined by dry weight of(More)
The soybean cyst nematode, Heterodera glycines, is one of the most economically important pathogens of soybean. Effective management of the nematode is often dependent on the planting of resistant soybean cultivars. During the past 40 years, more than 60 soybean genotypes and plant introductions (PI) have been reported as resistant to H. glycines. About 130(More)
Plant and soil nematodes significandy impact our lives. Therefore, we must understand and manage these complex organisms so that we may continue to develop and sustain our food production systems, our natural resources, our environment, and our quality of life. This publication looks specifically at soil and plant nematology. First, the societal impact of(More)
The complex nature of nematode-induced diseases of tobacco, the relationships between nematode levels and damage, the methods of determining these relationships, and the evolving nematode communities on tobacco in eastern North Carolina are described. Crop damage associated with these pathogens varies with nematode race and species, crop cultivar,(More)