Kenneth C. Norbury

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An experimental protocol was designed to assess humoral immune responses in animals antigenically challenged without the aid of adjuvants. The antigen selected was keyhole limpet hemocyanin (KLH). An enzyme-linked immunosorbent assay (ELISA) was utilized as the technique to measure serum antibody levels. The procedure was tested for sensitivity by use of a(More)
BACKGROUND Recent studies suggest that peritoneal fluid (PF) may be an important mediator of inflammation. The aim of this study was to test the hypothesis that PF may drive systemic inflammation in intra-abdominal sepsis by representing a priming agent for neutrophils. METHODS PF was collected 12 hours after the initiation of intra-abdominal sepsis in(More)
It has been reported that trypan blue treatment decreases the nonspecific resistance of mice to transplanted tumors and inhibits the in vitro cytotoxic activity of activated macrophages. We wished to determine whether this effect of trypan blue could be due to a selective inhibition of certain macrophage functions or whether it reflected a broader form of(More)
BACKGROUND We sought to determine the effect of peritoneal fluid from a novel animal model of abdominal compartment syndrome (ACS) on the proinflammatory status of polymorphonuclear leukocytes (PMNs) and monocytes. We hypothesize that peritoneal fluid is a potential priming and/or activating agent for PMNs/monocytes. METHODS ACS was induced in female(More)
In a swine model of ischemia/reperfusion injury coupled with sepsis, we have previously shown attenuation of secondary organ injury and decreased mortality with negative pressure therapy (NPT). We hypothesized that NPT modulates the intestinal microenvironment by mediating the innate immune system. Sepsis was induced in 12 anesthetized female pigs. Group 1(More)
BACKGROUND Negative pressure wound therapy (NPWT) has been used for complex sternotomy wounds. Some reports describe foam placement below the posterior sternal table. We compared the hemodynamic and pulmonary effects of foam location during NPWT after median sternotomy. METHODS Swine were randomized into four groups (n = 6 per group). A polyurethane open(More)
BACKGROUND : Current abdominal compartment syndrome (ACS) models rely on intraperitoneal instillation of fluid, air, and other space-occupying substances. Although this allows for the study of the effects of increased abdominal pressure, it poorly mimics its pathogenesis. We have developed the first reported large animal model of ACS incorporating(More)
The purpose of this large-animal study was to assess the safety and effects of negative pressure therapy (NPT) when used as temporary abdominal closure in the immediate post-decompression period after abdominal compartment syndrome (ACS). Using a hemorrhagic shock/resuscitation and mesenteric venous pressure elevation model, ACS was physiologically induced(More)
The effect of negative pressure therapy (NPT; The ABThera™ Open Abdomen Negative Pressure Therapy System, KCI USA, Inc., San Antonio, TX) on the integrity of small intestinal anastomoses was evaluated using in situ burst strength testing in a domestic pig model. In each of 3 swine, 8 anastomoses were created, 4 using sutures and 4 using staples. After 24(More)