Kenji Watanabe

Publications381
h-index46
Citations7,692
Highly Influential Citations237
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Chromatin-level regulation of biosynthetic gene clusters.
Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new…
Bacteriome-associated endosymbionts of the green rice leafhopper Nephotettix cincticeps (Hemiptera: Cicadellidae)
TLDR
In situ hybridization and transmission electron microscopy showed that the Sulcia symbiont and the β-proteobacterial symbionts are harbored within different types of bacteriocytes that constitute the outer and inner regions of the bacteriome, respectively.
Identification of a Novel Non-structural Maintenance of Chromosomes (SMC) Component of the SMC5-SMC6 Complex Involved in DNA Repair*
TLDR
It is concluded that Nse1p and the SMC5-SMC6 heterodimer together form a high molecular mass complex that is conserved in eukaryotes and required for both DNA repair and proliferation.
α-Glucosidase from a strain of deep-sea Geobacillus: a potential enzyme for the biosynthesis of complex carbohydrates
An α-glucosidase from Geobacillus sp. strain HTA-462, one of the deepest sea bacteria isolated from the sediment of the Mariana Trench, was purified to homogeneity and estimated to be a 65-kDa…
Molecular genetic mining of the Aspergillus secondary metabolome: discovery of the emericellamide biosynthetic pathway.
Triterpene Acids from the Leaves of Perilla frutescens and Their Anti-inflammatory and Antitumor-promoting Effects
TLDR
Eight triterpene acids isolated from ethanol extracts of the leaves of red and green perilla exhibited strong antitumor-promoting activity in an in vivo two-stage carcinogenesis test of mouse tumor by using 7,12-dimethylbenz(a)anthracene (DMBA) as an initiator and TPA as a promoter.
Differential Regulation of Rad18 through Rad6-dependent Mono- and Polyubiquitination*
TLDR
The results suggest that the amount of Rad18 in the nucleus is regulated differentially by mono- and polyubiquitination.
Enzymatic Conversion of Pheophorbide a to the Precursor of Pyropheophorbide a in Leaves of Chenopodium album
TLDR
It appears that the enzyme catalyzes the demethylation of the carbomethoxy group at C-13 2 of pheophorbide a by hydrolysis to yield methanol and the precursor, C-132carboxyl-pyropheoph orbide a, which is converted to pyropheophorbid a by spontaneous decarboxylation.
Enzymatic synthesis of aromatic polyketides using PKS4 from Gibberella fujikuroi.
TLDR
This work has demonstrated the E. coli expression and reconstitution of an iterative, unreduced fungal PKS, which selectively used octanoyl-CoA as the starter unit and synthesized two novel benzopyrone-containing polyketides.
Total biosynthesis of antitumor nonribosomal peptides in Escherichia coli
TLDR
The first example of the de novo total biosynthesis of biologically active forms of heterologous NRPs in Escherichia coli is shown, which can serve not only as an effective and flexible platform for large-scale preparation of natural products from simple carbon and nitrogen sources, but also as a general tool for detailed characterizations and rapid engineering of biosynthetic pathways for microbial syntheses of novel compounds and their analogs.
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