Kenji Konno

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OBJECTIVES To evaluate and describe the anti-herpesvirus effect of ASP2151, amenamevir, a novel non-nucleoside oxadiazolylphenyl-containing herpesvirus helicase-primase complex inhibitor. METHODS The inhibitory effect of ASP2151 on enzymatic activities associated with a recombinant HSV-1 helicase-primase complex was assessed. To investigate the effect on(More)
The highly potent and selective anti-DNA virus agent (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA] was found to inhibit in vitro the replication of a number of clinical varicella-zoster virus strains within the concentration range of 0.63—5.7 ng/ml. With a mean 50 % inhibitory concentration of 1.8 ng/ml and selectivity index of 29000,(More)
We have previously reported that ingenol derivatives are highly potent inhibitors of human immunodeficiency virus type 1 (HIV-1) replication in acutely infected cells. In this study, however, we have found that some ingenol derivatives strongly enhance the replication of HIV-1 in chronically infected cells at nanomolar concentrations. One of the derivatives(More)
1. Tryptic cleavage of fish myosin subfragment-1 (S-1) revealed its similar substructure of heavy chain to that of rabbit S-1. 2. The structural stability of fish S-1 was studied by thermal denaturation method, and a rapid polymerization of inactivated fish S-1, detected by turbidity increase, was characteristic. 3. The light-chain release and tryptic(More)
We have prepared chymotryptically split actin that retains the characteristic properties of intact actin. Chymotryptic digestion of G-actin produces an intermediate 35-kilodalton (kDa) fragment and from this a final product of 33 kDa known as the C-terminal "core". These fragments remain attached to an N-terminal 10-kDa fragment. The 35-kDa-10-kDa complex(More)
The chymotryptic digestion of G-actin in the presence of calcium produces not only a C-terminal 33 kDa "core," spanning from residue 68 to the C-terminus, but also an N-terminal Cys-10-containing fragment (10 kDa fragment), spanning from the N-terminus to the 44th residue. The minimum calcium concentration required for producing just these two structures is(More)
We present an experimental/theoretical study of the micro uidic electrophoresis of long DNA in self-assembling matrices of magnetic bead columns. Micro uidic technology proffers great potential for performing rapid, reproducible, inexpensive separations of DNA. In contrary to other separation methods, single molecule observations are possible during the(More)
The stabilizing effect of nucleotides against thermal denaturation of subfragment-1(S-1) was studied quantitatively. We showed that ATP- (ADP.P1) and AMPPNP-bound species are very stable, while ADP- and PPi-bound species are much less so (after ligand affinities are accounted for); these two stability classes correspond to the two CD and UD spectral(More)
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