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The growth kinetics in the presence of copper (Cu) of the protonema of the moss Scopelophila cataractae and the matrix polysaccharides of its cell walls have been analyzed in this study. Protonemal cells cultured in a medium containing 0.2mM CuSO(4) showed a rapid accumulation of Cu, reaching a maximum between 30 and 60d at approximately 65 micromolg(-1)(More)
A cell line of M. polymorpha was grown photoautotrophically in liquid suspension culture using 1% CO2 in air as sole carbon source. The growth rate in terms of cell dry-weight during the exponential phase was 0.171 and the doubling time was 1.76 d. The rate of increase in chlorophyll was 1.6 times higher than the growth rate. The highest content of(More)
The present work reports the results of a study on the growth kinetics and characterization of matrix polysaccharides in the cell walls of Lygodium japonicum prothallium grown in the presence of copper (Cu). When the prothallium was cultured in the media containing 0.2 mM or 0.4 mM CuSO(4), it showed a rapid accumulation of Cu with a maximum uptake of Cu(More)
A method to measure the concentrations of microcystins (MCs) in water samples has been developed by incorporating pre-column fluorescence derivatization and liquid chromatography (LC). A solid-phase extraction for pretreatment was used to extract the MCs in water samples. The MCs were derivatized with excimer-forming 4-(1-pyrene)butanoic acid hydrazide(More)
Growth and the production of volatile sesquiterpenoids by a chlorophyllous cell suspension culture from gametophytes of C. granulata, a leafy liverwort, were examined. Glucose was more effective than 2,4-dichlorophenoxyacetic acid for callus induction, and elimination of glucose from the medium resulted in prompt redifferentiation of plantlets. The cells(More)
Various specimen preparations for thin-section electron microscopy were tested to better preserve and visualize actin filaments in the cortex of the early embryos of Drosophila melanogaster. When embryos were treated with phalloidin prior to fixation, many actin filaments were observed in their cortex comparable to the staining with fluorescently labeled(More)
In Service Inspection by Acoustic Emission technique offers the user significant advantages, such as the capability of monitoring corrosion damage of the bottom plates of an oil tank without opening it. In this study, the mechanism of AE generation due to the corrosion of tank bottoms on strong acidic sand was examined. During the corrosion process, the(More)
The cDNA of extracellular alpha-l-arabinofuranosidase (alpha-l-AFase, EC secreted from suspension-cultured carrot cells (Daucus carota L. cv. Kintoki) was isolated and characterized. The nucleotide sequence of the cDNA (2.4 kb) revealed an open reading frame consisting of 655 amino acid residues. Sequence homology research showed 28.4% identity to(More)
A green-pigmented cell suspension culture of Marchantia polymorpha was established using the medium of Murashige and Skoog with addition of organic acids of the tricarboxylic-acid cycle, vitamins and sugars plus sugar alcohols, exclusion of kinetin, and replacement of sucrose with glucose. In continuous light, the cells grew exponentially for ca. 10 days;(More)
Exopolygalacturonase (exo-PGase, EC attacks the non-reducing terminus of the polygalacturonic acid in pectic molecules, releasing galacturonic acid. We cloned the cDNA of exo-PGase purified from cell homogenates of suspension-cultured carrot (Daucus carota L. cv. Kintoki) cells. The nucleotide sequence of the cDNA (1.4 kb) contains an open reading(More)