Kenichi Ogasawara

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Polymorphism of the ABO blood group gene was investigated in 262 healthy Japanese donors by a polymerase chain reactions-single-strand conformation polymorphism (PCR-SSCP) method, and 13 different alleles were identified. The number of alleles identified in each group was 4 for A1 (provisionally called ABO*A101, *A102, *A103 and *A104 according to the(More)
We identified five different alleles, tentatively named ABO*O301, *O302, *R102, *R103, and *A110, in Japanese individuals possessing the blood group O phenotype. These alleles lack the guanine deletion at nucleotide position 261 which is shared by a majority of O alleles. Nucleotide sequence analysis revealed that *O301 and *O302 had single nonsynonymous(More)
BACKGROUND The ABO blood group is important in blood transfusion. Recently, an erythroid cell-specific regulatory element has been identified in the first intron of ABO using luciferase reporter assays with K562 cells. The erythroid cell-specific regulatory activity of the element was dependent upon GATA-1 binding. In addition, partial deletion of Intron 1(More)
1Department of Legal Medicine, Gunma University Graduate School of Medicine, Maebashi, Japan; 2Cancer Chemotherapy Center and Hematology, University of Occupational and Environmental Health, Kitakyushu, Japan; 3Department of Legal Medicine, Shimane University School of Medicine, Izumo, Japan; 4Division of Medical Genetics and Biochemistry, Faculty of(More)
The human ABO blood group system is of great importance in blood transfusion and organ transplantation. The ABO system is composed of complex carbohydrate structures that are biosynthesized by A- and B-transferases encoded by the ABO gene. However, the mechanisms regulating ABO gene expression in epithelial cells remain obscure. On the basis of DNase(More)
The ABO blood group is of great importance in blood transfusion and organ transplantation. However, the mechanisms regulating human ABO gene expression remain obscure. On the basis of DNase I-hypersensitive sites in and upstream of ABO in K562 cells, in the present study, we prepared reporter plasmid constructs including these sites. Subsequent luciferase(More)
Introduction Discrepancy of results of ABO groups between red cell testing and serum testing is a significant issue in transfusion medicine1. One of its reported causes is blood chimerism, which shows mixed-field agglutination. However, mixed-field agglutination can be seen in various situations, including ABO-incompatible stem cell transplantation, recent(More)
T he Lan antigen and antibody (anti-Lan) were first reported by van der Hart and colleagues in a severe hemolytic transfusion reaction case. The Lan− phenotype is very rare worldwide and is usually identified through the presence of anti-Lan. The Lan− phenotype has been reported in the black, Caucasian, and Japanese populations. The Japanese Red Cross Blood(More)
A 6 0 is clinically the most important blood group system in transfusion medicine and includes many variant phenotypes. To understand the molecular genetic basis of this polymorphic system, we have analyzed genomic DNAs obtained from Japanese individuals possessing variant AB0 phenotypes including A,, Ax, A,,, cis-AB, B., and B-,. By polymerase chain(More)
BACKGROUND In(Lu) is characterized by a reduced expression of antigens in the Lutheran blood group system as well as other blood group antigens. Mutations of the erythroid transcription factor, KLF1, have been reported to cause the In(Lu) phenotype, and we investigated Japanese In(Lu) to estimate the prevalence of the phenotype and KLF1 polymorphism. (More)