Keng-Ming Chang

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The Saccharomyces cerevisiae 2-μm plasmid is a multicopy selfish genome that resides in the nucleus. The genetic organization of the plasmid is optimized for stable, high-copy propagation in host-cell populations. The plasmid's partitioning system poaches host factors, including the centromere-specific histone H3-variant Cse4 and the cohesin complex,(More)
The basic networking unit in Bluetooth is piconet, and a larger-area Bluetooth network can be formed by multiple piconets, called scatternet. However, the structure of scat-ternets is not defined in the Bluetooth specification and remains as an open issue at the designers' choice. It is desirable to have simple yet efficient scatternet topologies with well(More)
The multi-copy 2 micron plasmid of Saccharomyces cerevisiae, a resident of the nucleus, is remarkable for its high chromosome-like stability. The plasmid does not appear to contribute to the fitness of the host, nor does it impose a significant metabolic burden on the host at its steady state copy number. The plasmid may be viewed as a highly optimized(More)
The basic networking unit in Bluetooth is piconet, and a larger-area Bluetooth network can be formed by multiple piconets, called scatternet. However, the structure of scatternets is not defined in the Bluetooth specification and remains as an open issue at the designers' choice. It is desirable to have simple yet efficient scatternet topologies with good(More)
The Helicobacter pylori (Hp) Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (AdT) plays important roles in indirect aminoacylation and translational fidelity. AdT has two active sites, in two separate subunits. Kinetic studies have suggested that interdomain communication occurs between these subunits; however, this mechanism is not well understood. To(More)
The partitioning locus STB of the selfish plasmid, the 2μm circle, of Saccharomyces cerevisiae is essential for the propagation of this multi-copy extra-chromosomal DNA element with nearly chromosome-like stability. The functional competence of STB requires the plasmid-coded partitioning proteins Rep1 and Rep2 as well as host-coded proteins. Host factors(More)
This study investigates the leg crossing effect originated from meditation. We use pulse transit time as index for this study. Pulse transit time is measured form the time interval between ECG R peak position and the corresponding pulse peak. There are 14 subjects participated in this study and two half lotus leg crossing postures and one free leg stretch(More)
Accurate aminoacylation of tRNAs by the aminoacyl-tRNA synthetases (aaRSs) plays a critical role in protein translation. However, some of the aaRSs are missing in many microorganisms. Helicobacter pylori does not have a glutaminyl-tRNA synthetase (GlnRS) but has two divergent glutamyl-tRNA synthetases: GluRS1 and GluRS2. Like a canonical GluRS, GluRS1(More)
The yeast 2-micron plasmid epitomizes the evolutionary optimization of selfish extra-chromosomal genomes for stable persistence without jeopardizing their hosts' fitness. Analyses of fluorescence-tagged single-copy reporter plasmids and/or the plasmid partitioning proteins in native and non-native hosts reveal chromosome-hitchhiking as the likely means for(More)
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