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The action of the cell-wall-associated proteinases from Lactococcus lactis subsp. cremoris strains H2 and SK112 on bovine beta-casein was compared. The proteinase from the H2 strain was characterised as a PI-type proteinase since it did not hydrolyse alpha s1-casein and the initial trifluoroacetic acid-soluble products of beta-casein hydrolysis were(More)
We describe here the cloning and complete sequence analysis of the rearranged kappa variable region gene from the V kappa-1A-producing myeloma (C.AL20-TEPC-105). A 5'-flanking region probe from the V kappa-1A gene has been used to study the V kappa-1 germ-line gene family in strains of mice differing at the Ig kappa-Ef2 locus. All Ef2a strains examined(More)
Germ line genes encoding mouse Ig kappa-chains belonging to the V kappa-1 group have been isolated from BALB/c, NZB, and CE, three inbred strains of differing kappa haplotype. The V kappa-1A and V kappa-1C germ line genes isolated from BALB/c (Ig kappa c) were identical to those previously described. These are the two major V kappa-1 germ line genes in(More)
Expressed sequence tags (ESTs) are short single pass sequence reads derived from cDNA libraries, they have been used for gene discovery, detection of splice variants, expression of genes and also transciptome analysis. Clustering of ESTs is a vital step before they can be processed further. Currently there are many EST clustering algorithms available.(More)
DNA database consists of many nucleotide sequences, it is not only supporting typical database queries, but it also needs to facilitate sequence search and alignment. In this paper, we present an object-oriented nucleotide database which is designed not only for the convenience of executing normal database operations such as insertion, modification or data(More)
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