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The docking and fusion of synaptic vesicles with the presynaptic plasma membrane require the interaction of the vesicle-associated membrane protein VAMP with the plasma membrane proteins syntaxin and SNAP-25. Both of these proteins behave as integral membrane proteins, although they are unusual in that they insert into membranes post-translationally.(More)
documented for switchgrass and big bluestem (Perry and Baltensperger, 1979; George and Hall, 1983; Griffin Predicting forage quality would help producers schedule hay harand Jung, 1983; Mitchell et al., 1994a). However, most vesting to obtain desired hay quality. Our objective was to determine if growing degree day (GDD), day of the year (DOY), mean stage(More)
The neuronal presynaptic membrane t-SNARE complex consists of the transmembrane protein syntaxin with the palmitoylated protein SNAP-25. In non-neuronal tissues, SNAP-23 replaces SNAP-25 in the t-SNARE complex, although the mechanism of membrane anchoring of SNAP-23 has not been determined. We now report that like SNAP-25, SNAP-23 is palmitoylated in vivo(More)
The mutagenicity of r-8,t-9-dihydroxy-t-10, 11-oxy-8,9,10,11-tetrahydrobenz[a]anthracene (BA-8,9-diol 10, 11-oxide) toward Salmonella typhimurium TA 100 is not decreased by the presence of large amounts of highly purified microsomal or cytosolic epoxide hydrolase. However, highly purified dihydrodiol dehydrogenase inactivates this diol epoxide, which is a(More)
The adaptive advantage of epibenthic articulate brachiopods over inarticulate forms resulted from a modification of the mechanics of shell opening from an indirect hydraulic system to a direct muscular one. As a consequence, the articulate brachiopods were able to reduce the complex muscular system of the ancestral inarticulates, freeing two-thirds of the(More)
A method is described for the purification of an enzyme, which catalyzes the conversion of benzene dihydrodiol to catechol, from rat liver cytoplasmic fraction to apparent homogeneity. The purification involved (NH4)2SO4 fractionation, DEAE-cellulose chromatography, interfacial salting-in and gel filtration through Sephadex G-100 superfine. The end product,(More)