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Adult mesenchymal stem cells secrete a variety of angiogenic cytokines and growth factors, so we proposed that these paracrine mechanisms may be used to promote vascularization and growth for tissue engineering in vivo. We tested whether or not human adipose-derived stem cells (ASCs) promote tissue formation in rats. ASCs were evaluated in vitro for mRNA(More)
We have previously established a chamber model of tissue engineering that promotes de novo angiogenesis and vascularization of engrafted cells and tissues when combined with an extracellular matrix. Here we demonstrate that the mouse chamber (MC) model can sustain ectopic grafts of murine fetal thymus lobes and, to a limited degree, human pediatric thymus(More)
The present study was performed to check the viability of eggs, filariform larvae and adults of Strongyloides venezuelensis exposed to various conditions for an in vitro maintenance. The eggs in the feces remained viable for about 25 days at 4 degrees C and 15 days at room temperature. However, the isolated eggs in sterile saline lost their viability within(More)
Cardiac tissue engineering offers promise as a surgical approach to cardiac repair, but requires an adequate source of cardiomyocytes. Here we evaluate the potential for generating human cardiac muscle cells in vivo from adipose-derived stem cells (ASC) by co-implanting in a vascularised tissue engineering chamber with inducing rat cardiomyocytes (rCM).(More)
Flashbulb memories are vivid memories of the details surrounding the discovery of an emotional event. We investigated whether the nuclear accident that occurred in Japan in 1999 produced flashbulb memories among people who lived near the accident site. A questionnaire was distributed twice (approximately 3 weeks after the accident and 1 year later) to (1)(More)
Manganese-enhanced magnetic resonance imaging (MEMRI) was used to investigate retrograde axonal tracing in the rat sciatic nerve model to assess its potential to examine peripheral nerve injury. The right sciatic nerve was exposed and crushed. After each recovery period, the distal part of the right sciatic nerve was injected with manganese (400 mM, 15(More)
This study examined the influence of familiarity and novelty on the mere exposure effect while manipulating the presentation of background information. We selected presentation stimuli that integrated cars and backgrounds based on the results of pilot studies. During the exposure phase, we displayed the stimuli successively for 3 seconds, manipulating the(More)
In this study, we focused on the influence of indoor thermal comfort on the mere exposure effect while manipulating the Predicted Mean Vote (PMV). We used neutral random shapes as the stimuli and controlled exposure frequency (0, 1, 5, 10, and 15 times) and PMV (+2=hot,+1=warm,0,-1=cool -2=cold). After the acclimation phase, participants were exposed to(More)
The effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae (L3) to rats were investigated. A significantly higher body length was observed in L3 from filter paper culture (597.3 +/- 32.2 microns) than those in fecal (509.9 +/- 35.0 microns) and nutrient broth culture (503.3 +/- 31.0(More)