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Genomic DNA from 19 Japanese patients with congenital lipoid adrenal hyperplasia (lipoid CAH) representing 16 different families was examined to identify the genetic alterations of steroidogenic acute regulatory protein (StAR). Ten of 19 patients had a 46,XX karyotype and nine had a 46,XY karyotype. Six of the 46,XX patients have experienced spontaneous(More)
We previously reported Thermus aquaticus DNA polymerase (Taq) and T. thermophilus DNA polymerase (Tth) synthesized poly(dA-dT) at 65°C in the apparent absence of primer/template (2,3). The polymerase preparations were highly purified enzymes provided by various manufacturers such as Invit-rogen, Roche Molecular Biochemicals, Toyobo, etc. We examined here(More)
A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivity and quantitative capacity of the assay were validated(More)
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