Kelly M Marno

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The discovery of novel anti-viral restriction factors illuminates unknown aspects of innate sensing and immunity. We identified RNA-associated Early-stage Anti-viral Factor (REAF) using a whole genome siRNA screen for restriction factors to human immunodeficiency virus (HIV) that act in the early phase of viral replication. We observed more than 50 fold(More)
The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination(More)
Background The Polymerase II-Associated Factor 1 (PAF1) is a cellular protein with essential roles in transcription activation and repression, polyA tail cleavage, regulation of RNA polymerase elongation, regulation of gene expression, cell cycle control and mRNA quality surveillance[1]. It is part of a multicomponent complex together with other cellular(More)
Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication in human cells is restricted at early postentry steps by host inhibitory factors. We previously described and characterized an early-phase restriction of HIV-1 and -2 replication in human cell lines, primary macrophages, and peripheral blood mononuclear cells. The(More)
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