Keisuke Tomioka

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Many bands were detected on an electrophoretic profile of double-stranded (ds) RNA preparation from a single strain of Fusarium poae isolated from wheat. When the purified dsRNA sample was deep-sequenced by a next-generation sequencer, sixteen virus-like assembled contigs with predicted amino acid sequences showing homologies to respective viral(More)
Based on inoculation experiments and morphological studies on the pathogenic isolates of Plectosporium spp., Microdochium blight (Hakuhan-byo in Japanese) of pumpkin (Cucurbita maxima Duch.) occurring in Japan was reconfirmed to be caused by Plectosporium tabacinum, and seedling blight (Kabugare-byo in Japanese) of ranunculus (Ranunculus asiaticus L.) was(More)
AIMS To investigate the prevalence of culturable and nonculturable Legionella species in hot water systems of public buildings in Japan and assess the risk factors associated with Legionella contamination in hot water systems. METHODS AND RESULTS Legionella species were detected by conventional culture and molecular methods in 130 water samples collected(More)
Severe spotting and blight of leaves caused by Colletotrichum destructivum were found on snapdragon (Antirrhinum majus L.), a scrophulariaceous ornamental, in open fields in Shizuoka Prefecture, Japan, from June through September 2004. The fungus is added to the group of the pathogens causing anthracnose of snapdragon.
In some areas of Japan, yellow spots with white pustules on leaves, stems, petioles, peduncles and calyces were found on Ipomoea nil, I. triloba, I. lacunosa and I. hederacea var. integriuscula. We demonstrated that the diseases on I. nil, I. triloba and I. lacunosa were caused by host-specific strains of Albugo ipomoeae-panduratae and defined three forma(More)
The National Institute of Agrobiological Sciences (NIAS) is implementing the NIAS Genebank Project for conservation and promotion of agrobiological genetic resources to contribute to the development and utilization of agriculture and agricultural products. The project's databases (NIASGBdb; http://www.gene.affrc.go.jp/databases_en.php) consist of a genetic(More)
Restriction landmark genome scanning (RLGS) is a 2-DE of genomic DNA, which visualizes thousands of loci. In a conventional RLGS method for methylation analysis, we have used a methylation sensitive restriction enzyme, NotI as a landmark. However, it was unable to discriminate methylation polymorphism from sequence polymorphism. Here, we report an improved(More)
A severe rot of postharvest fruits of sweet pepper, a variety of Capsicum annuum, was found in Kagawa Prefecture in southwestern Japan in August 1999. A fungus, isolated repeatedly from the diseased fruits and identified as Stemphylium lycopersici, was demonstrated to be pathogenic to fruits of sweet pepper. The disease was new to Japan, and the fungus was(More)
Severe wilt with spots and/or leaf and stem blight were found on a scrophulariaceous flowering plant, Nemesia strumosa, grown in Kagawa Prefecture, Japan, in February 1999. Wilted plants had numerous lesions and died early. A mitosporic fungus isolated repeatedly from the diseased plants was identified as Colletotrichum fuscum and was demonstrated to cause(More)
Severe spotting and blighting of leaves were found on bacopa (Sutera cordata), a scrophulariaceous ornamental, in greenhouses in Gunma Prefecture, Japan, from January through February 2007. After we isolated and identified the causal fungus as Colletotrichum destructivum and inoculated host plants with the isolate to confirm pathogenicity, we named this new(More)