Keiji Watabe

  • Citations Per Year
Learn More
We report the isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica). These markers were tested in 24 individuals each collected from three distinct populations in Ichikai-machi, Tochigi Prefecture. The number of observed alleles per locus ranged from 3 to 24 across all populations, and the(More)
Twenty-three polymorphic microsatellite markers for Sympetrum frequens were developed and characterized. The number of distinct alleles per locus in 32 individuals ranged from 2 to 23. The observed heterozygosity ranged from 0.031 to 0.938, while the expected heterozygosity varied from 0.031 to 0.922. No loci deviated significantly from Hardy–Weinberg(More)
We have developed microsatellite DNA markers for Mesopodopsis orientalis (Tattersall 1908), a widely distributed mysid crustacean in shallow waters of the coastal and estuarine systems across India to Indonesia and the Philippines. A total of 18 novel polymorphic microsatellite markers were identified in 32 individuals from a sandy beach at Teluk Kumbar in(More)
Land improvements in Japan involve the need to conserve ecosystems and biodiversity, hence research and research methods related to ecosystem conservation in rural aquatic areas are increasing and developing. Analysis in rural aquatic ecosystems using stable carbon and nitrogen isotope ratios is an important research method and such isotope ratios are used(More)
Rhinogobius sp. YB (Gobiidae) distributed in the Ryukyu Archipelago is a fluvial species of gobies. We present 34 novel microsatellite loci that were tested on 32 individuals from the Okierabu-jima Island in Kagoshima Prefecture. Seventeen loci were polymorphic, while the remaining 17 were monomorphic. The number of alleles per the polymorphic loci ranged(More)
Thirty-two polymorphic microsatellite loci were indentified and characterized from the mysid Crustacea Mesopodopsis tenuipes Hanamura et al., 2008. The number of observed alleles per locus in 32 individuals collected from its type locality in Malaysia ranged from 2 to 22. The observed heterozygosity ranged from 0.031 to 0.906, while the expected(More)
We developed and characterized a total of 19 novel polymorphic microsatellite loci from the genome of Clupeichthys aesarnensis. Flanking PCR primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 30 individuals ranging from two to 15. The observed heterozygosity ranged from 0.033 to 0.933,(More)
Twenty one polymorphic microsatellite loci for the Japanese dace (Tribolodon hakonensis) were isolated and characterized. The number of observed alleles per locus in 32 individuals ranged from 3 to 30. The observed and expected heterozygosities ranged from 0.125 to 0.969 and from 0.175 to 0.973, respectively. All loci conformed to Hardy–Weinberg(More)
We isolated and characterized a total of 40 polymorphic microsatellite loci from the genome of Parambassis siamensis. Flanking Polymerase Chain Reaction primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 32 individuals ranging from 2 to 29. The observed heterozygosity ranged from 0.063 to(More)
Fecal DNA samples from the red-eared slider and Reeves’ pond turtle, suspected pests of lotus root paddies, were used to identify the plant species eaten by these turtles in order to develop a strategy for rural ecosystem conservation. The fecal samples were obtained from young and adult individuals (mostly female) of both species living in agricultural(More)