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PURPOSE To compare the relative degrees of adherence of a clinical strain of Pseudomonas aeruginosa to the optic material of four intraocular lenses (IOLs). SETTING Center for Applied and Environmental Microbiology, Georgia State University, Atlanta, Georgia, USA. METHODS Intraocular lens optics made of poly(methyl methacrylate) (PMMA), AcrySof-acrylic,(More)
This study used video time-lapse recording to characterize the dynamic features of corneal epithelial cells and keratocytes during in vitro wound closure. Confluent cultures of these two cell types from rabbits were established in Rose chambers. A wound 4 or 10 mm in diameter was produced in the center of each culture by mechanical removal of cells. Wound(More)
Epithelial neuronotropic factor (ENF) is secreted by cultured epithelial cells of rabbit cornea and conjunctiva, and is active in promoting survival and inducing neurite outgrowth of cultured trigeminal neurons. This study evaluated the relation of ENF to corneal nerve regeneration utilizing a model of heptanol-induced epithelial wounding. The organ culture(More)
The three cell types of rabbit cornea (epithelium, stromal fibroblasts and endothelium) were isolated by an improved method using both microdissection and selective enzyme treatment. This technique reproducibly resulted in an almost total recovery of each cell type from a given cornea. When maintained in culture, the three cell types showed different(More)
PURPOSE To quantify the surface retention of several ophthalmic viscoelastic agents following irrigation and aspiration (I/A) using a new in vitro method. SETTING Alcon Laboratories, Inc., Fort Worth, Texas, USA. METHODS A rabbit corneal endothelial cell line was cultured to confluency in 24-well plates, and the cells were labeled quantitatively with(More)
This study used a transcorneal freezing technique to produce a 2-mm circular, central wound in the rabbit cornea for investigating corneal nerve regeneration. All the corneal cells, nerves, and associated Schwann cells were dead inside the wound, but the extracellular matrix components remained intact. The destroyed epithelium and endothelium were replaced(More)
PURPOSE To quantitatively determine the cohesion of ophthalmic viscoelastic agents using an in vitro method based on dynamic aspiration kinetics. SETTING Alcon Laboratories, Inc., Fort Worth, Texas, USA. METHOD Five viscoelastic agents were tested: Healon GV (sodium hyaluronate 1.4%); Provisc (sodium hyaluronate 1.0%); Healon (sodium hyaluronate 1.0%);(More)
This study utilized peroxidase cytochemical methods to survey and analyze the distribution, morphology, and functional relationship of the various components of sensory nerve endings in a focal region of the anterior hard palate (incisive papilla) of the rat. The studies included an anatomical survey of this relatively unknown oral structure, confirmation(More)