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Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that(More)
STUDY DESIGN Retrospective analysis of adjacent disc degeneration (ADD) after anterior cervical decompression and fusion (ADF). OBJECTIVES To elucidate the influence of the number of levels fused in ADF on the incidence of ADD. SUMMARY OF BACKGROUND DATA ADD is known as a complication associated with ADF. However, how the number of levels fused affects(More)
STUDY DESIGN The reliabilities of and correlations among 5 standard methods of assessing cervical sagittal alignment were evaluated. OBJECTIVE To investigate the reliabilities of and correlations among 5 standard methods of assessing cervical sagittal alignment. SUMMARY OF BACKGROUND DATA Although various cervical sagittal alignment assessment methods(More)
STUDY DESIGN In vitro study on the effects of pulsed low intensity ultrasound on the cellular metabolism of bovine intervertebral disc cells. OBJECTIVE To determine whether pulsed low intensity ultrasound has effects on cell proliferation and extracellular matrix metabolism by bovine intervertebral disc cells. SUMMARY OF BACKGROUND DATA The application(More)
We report a case of an 80-year-old woman with dropped head syndrome associated with cervical spondylotic myelopathy. She could not keep her cervical spine in a neutral position for >1 minute. She had a disturbed gait and severe kyphotic deformity in her thoracic spine. Magnetic resonance imaging revealed severe compression of the spinal cord due to cervical(More)
Proper regulation of transcription is essential for cells to acquire and maintain cell identity. Transcriptional activation plays a central role in gene regulation and can be modulated by introducing transcriptional activators such as transcription factors. Activators act on their specific target genes to induce transcription. Reprogramming experiments have(More)
It is known that differentiated cells can be reprogrammed to an undifferentiated state in oocyte cytoplasm after nuclear transfer. Recently, some reports suggested that Xenopus egg extracts have the ability to reprogram mammalian somatic cells. Reprogramming events of mammalian cells after Xenopus egg extract treatment and after cell culture of(More)
STUDY DESIGN A study was performed using an axial loading device in healthy young subjects. OBJECTIVE To determine whether sagittal alignment during axial loading using a compression device can accurately simulate the standing posture. SUMMARY OF BACKGROUND DATA Axial compression devices are widely used for simulation of standing position during(More)
Differentiated cells can be experimentally reprogrammed back to pluripotency by nuclear transfer, cell fusion or induced pluripotent stem cell technology. Nuclear transfer and cell fusion can lead to efficient reprogramming of gene expression. The egg and oocyte reprogramming process includes the exchange of somatic proteins for oocyte proteins, the(More)
Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization and in somatic nuclei after somatic cell nuclear transfer. This ability of eggs and oocytes is essential for normal development. Nuclear actin and actin-binding proteins have been shown to contribute to transcription, although their mode of action is(More)