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The breast and ovarian cancer suppressor BRCA1 acquires significant ubiquitin ligase activity when bound to BARD1 as a RING heterodimer. Although the activity may well be important for the role of BRCA1 as a tumor suppressor, the biochemical consequence of the activity is not yet known. Here we report that BRCA1-BARD1 catalyzes Lys-6-linked polyubiquitin(More)
The possible function of the alpha-inc allele (an alpha mating-type allele that is insensitive to the function of the homothallic gene system) was investigated by means of protoplast fusion. The fusion of protoplasts prepared from haploid strains of alpha-inc HO HMalpha HMa and alpha ho hmalpha HMa gave rise mainly to nonmating clones (58 of 64 isolates)(More)
To investigate the dominance and recessiveness of the homothallism genes, HMalpha/hmalpha and HMa/hma, for mating-type conversion, we constructed hybrids with various configurations of the homothallic genes by fusion of protoplasts prepared from haploid strains having identical mating types. Eight different combinations of the homothallic genes were tested(More)
A composite plasmid (pAT2010) has been constructed in vitro from RSF2124 and Bacillus subtilis IFO3022 plasmid (pAT1060) by covalent joining of the two DNA molecules by means of Escherichia coli DNA ligase through the cohesive ends generated by restriction endonuclease RI (EcoRI) cleavage. The composite plasmid was selected by transformation of E. coli C600(More)
p-Hydroxybenzoate hydroxylase (EC from Pseudomonas desmolytica is known to form a stable complex with its substrate. Investigations were made on properties of the complex by spectrophotometric measurements and photooxidation. The addition ofp-hydroxybenzoate or benzoate to the oxidized (with respect to bound FAD) enzyme solution caused changes in(More)
p-Hydroxybenzoate hydroxylase (EC was rapidly inactivated by treatment with phenylglyoxal, by a process obeying pseudo-first order kinetics. The reaction with the reagent was also examined by amino acid analyses, radioactivity measurements, and spectrophotometric analyses. Results of these analyses were consistent with each other, which shows(More)
Host specific restriction was detected in 13 Bacillus strains, when 63 strains of Bacillus subtilis and 15 other Bacillus strains were tested with phage Φ105C. These 13 strains were classified into 8 groups (M,H,C,N,E,F,G,P) by the type of restriction. M-type strains (B. subtilis Marburg 168, its derivatives, and two other strains) showed relatively weak(More)
Soil-isolated alkalophilic streptomycetes were found to produce antimycin A with high frequency. One of the alkalophilic strains, strain No. 1543, produced a significant amount of antimycin A at high alkaline pH. Taxonomical studies revealed the strain wasStreptomyces baarnensis subsp.okayamanensis subsp. nov.
  • Kei Arima
  • Journal of Industrial Microbiology
  • 2005
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