Kee Pum Kim
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Characterization of intein homing endonuclease encoded in the DNA polymerase gene of Thermococcus marinus.
- Heejin Bae, K. P. Kim, J. M. Song, J. Kim, J. S. Yang, S. T. Kwon
- Biology, Medicine
- FEMS microbiology letters
- 1 August 2009
The DNA polymerase gene of Thermococcus marinus (Tma) contains an intein inserted at the pol-b site that possesses a 1611-bp ORF encoding a 537-amino acid residue. The LAGLIDADG motif, often found in… Expand
Characterization of DNA polymerase from the hyperthermophilic archaeon Thermococcus marinus and its application to PCR
The family B DNA polymerase gene from the archaeon Thermococcus marinus (Tma) contains a long open reading frame of 3,939 bp that encodes 1,312 amino acid residues. The gene is split by one… Expand
Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A2 (hVPLA2).
Secretory phospholipase A2 (PLA2) consists of several 14-kDa isoforms with extensive homology, which makes it difficult to identify a specific isoform. In this study, we have developed and… Expand
Characterization and PCR application of a new high-fidelity DNA polymerase from Thermococcus waiotapuensis.
- Sung Suk Cho, K. P. Kim, Kang Keun Lee, Man-Hui Youn, S. Kwon
- Medicine, Chemistry
- Enzyme and microbial technology
- 10 December 2012
The family B DNA polymerase gene from the euryarchaeon Thermococcus waiotapuensis (Twa) contains an open reading frame of 4404 bases that encodes 1467 amino acid residues. The gene is split by two… Expand
Improved thermostability and PCR efficiency of Thermococcus celericrescens DNA polymerase via site-directed mutagenesis.
- K. P. Kim, Sung Suk Cho, Kang Keun Lee, Man Hui Youn, S. Kwon
- Biology, Medicine
- Journal of biotechnology
- 10 September 2011
The Thermococcus celericrescens (Tcel) DNA polymerase gene, which contains a 2328-bp open reading frame that encodes 775 amino acid residues, was expressed in the Escherichia coli strain… Expand
Cloning, expression, and PCR application of DNA polymerase from the hyperthermophilic archaeon, Thermococcus celer
The family B DNA polymerase gene was amplified from Thermococcus celer genomic DNA by using the degenerate primers and DNA walking PCR. The Tce DNA polymerase gene was cloned and sequenced. The gene… Expand
2 GLOBAL PEPTIDE SEQUENCING AND QUANTIFICATION OF PROTEINS IN PORCINE PARTHENOTES BY PROTEOMICS
Mass spectrometry (MS) is a powerful emerging tool in proteomics for global identification and quantification of proteins and their differential analysis. The aim of this study was to utilize MS for… Expand