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  • Ke Hu, Jeff Johnson, Laurence Florens, Martin Fraunholz, Sapna Suravajjala, Camille DiLullo +3 others
  • 2006
The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular(More)
—Cochannel (two-talker) speech separation is predominantly addressed using pretrained speaker dependent models. In this paper, we propose an unsupervised approach to separating cochannel speech. Our approach follows the two main stages of computational auditory scene analysis: segmentation and grouping. For voiced speech segregation, the proposed system(More)
Toxoplasma gondii is an obligatory intracellular parasite, an important human pathogen, and a convenient laboratory model for many other human and veterinary pathogens in the phylum Apicomplexa, such as Plasmodium, Eimeria, and Cryptosporidia. 22 subpellicular microtubules form a scaffold that defines the cell shape of T. gondii. Its cytoskeleton also(More)
Viruses initiate infection by attaching to molecules or receptors at the cell surface. Hepatitis C virus (HCV) enters cells via a multistep process involving tetraspanin CD81, scavenger receptor class B member I, and the tight junction proteins Claudin-1 and Occludin. CD81 and scavenger receptor class B member I interact with HCV-encoded glycoproteins,(More)
—While a lot of effort has been made in computational auditory scene analysis to segregate voiced speech from monaural mixtures, unvoiced speech segregation has not received much attention. Unvoiced speech is highly susceptible to interference due to its relatively weak energy and lack of harmonic structure, and hence makes its segregation extremely(More)
The apicomplexans are a large group of parasitic protozoa, many of which are important human and animal pathogens, including Plasmodium falciparum and Toxoplasma gondii. These parasites cause disease only when they replicate, and their replication is critically dependent on the proper assembly of the parasite cytoskeletons during cell division. In addition(More)
Toxoplasma gondii is a leading cause of congenital birth defects, as well as a cause for ocular and neurological diseases in humans. Its cytoskeleton is essential for parasite replication and invasion and contains many unique structures that are potential drug targets. Therefore, the biogenesis of the cytoskeletal structure of T. gondii is not only(More)
—Singing pitch estimation and singing voice separation are challenging due to the presence of music accompaniments that are often nonstationary and harmonic. Inspired by computational auditory scene analysis (CASA), this paper investigates a tandem algorithm that estimates the singing pitch and separates the singing voice jointly and iteratively. Rough(More)
Glutathione peroxidase-1 (GPx1) is a pivotal intracellular antioxidant enzyme that enzymatically reduces hydrogen peroxide to water to limit its harmful effects. This study aims to identify a microRNA (miRNA) that targets GPx1 to maintain redox homeostasis. Dual luciferase assays combined with mutational analysis and immunoblotting were used to validate the(More)