Kazuyuki Nakamura

Yasuhiro Kuramitsu9
Takao Kitagawa8
Junko Akada6
Kazuhiro Tokuda6
9Yasuhiro Kuramitsu
8Takao Kitagawa
6Junko Akada
6Kazuhiro Tokuda
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Heterotrimeric G proteins, composed of α, β, and γ subunits, can transduce a variety of signals from seven-transmembrane-type receptors to intracellular effectors. By whole-exome sequencing and subsequent mutation screening, we identified de novo heterozygous mutations in GNAO1, which encodes a Gαo subunit of heterotrimeric G proteins, in four individuals(More)
The aim of this paper is to demonstrate the potential power of large-scale particle filtering for the parameter estimations of in silico biological pathways where time course measurements of biochemical reactions are observable. The method of particle filtering has been a popular technique in the field of statistical science, which approximates posterior(More)
Data assimilation is a method of combining an imperfect simulation model and a number of incomplete observation data. Sequential data assimilation is a data assimilation in which simulation variables are corrected at every time step of observation. The ensemble Kalman filter is developed for a sequential data assimilation and frequently used in geophysics.(More)
—In this paper, we develop a new generic implementation scheme for numerical smoothing in nonlinear and Bayesian state–space modeling. Our new generic implementation scheme, which we call recursive recomputation scheme, reduces the space complexity from () to (log), at the cost of (log) times computation of filtering distributions in time complexity. This(More)
  • Yufeng Wang, Yasuhiro Kuramitsu, Kazuhiro Tokuda, Byron Baron, Takao Kitagawa, Junko Akada +4 others
  • 2014
Poly (ADP-ribose) polymerase-1 (PARP-1) and autophagy play increasingly important roles in DNA damage repair and cell death. Gemcitabine (GEM) remains the first-line chemotherapeutic drug for pancreatic cancer (PC). However, little is known about the relationship between PARP-1 expression and autophagy in response to GEM. Here we demonstrate that GEM(More)
  • Junko Akada, Masumi Okuda, Narumi Hiramoto, Takao Kitagawa, Xiulian Zhang, Shuichi Kamei +8 others
  • 2014
Serum antibodies against pathogenic bacteria play immunologically protective roles, and can be utilized as diagnostic markers of infection. This study focused on Japanese child serum antibodies against Helicobacter pylori, a chronically-infected gastric bacterium which causes gastric cancer in adults. Serological diagnosis for H. pylori infection is well(More)
Different models for animal cell cytokinesis posit that the stiffness of the equatorial cortex is either increased or decreased relative to the stiffness of the polar cortex. A recent work has suggested that the critical cytokinesis signaling complex centralspindlin may reduce the stiffness of the equatorial cortex by inactivating the small GTPase Rac. To(More)
  • Junko Akada, Shuichi Kamei, Akane Ito, Moe Ito, Takao Kitagawa, Hiroko Furumoto +8 others
  • 2012
We report here a new type of protein chip to detect antibodies in sera. This chip method was used to a prototype created to detect hepatocellular carcinoma (HCC) -related autoantibodies in the sera of hepatitis C virus (HCV) infected individuals. Five cysteine-tagged (Cys-tag) and green fluorescent protein (GFP)-fused recombinant heat shock protein 70(More)
De novo GNAO1 variants have been found in four patients including three patients with Ohtahara syndrome and one patient with childhood epilepsy. In addition, two patients showed involuntary movements, suggesting that GNAO1 variants can cause various neurological phenotypes. Here we report an additional four patients with de novo missense GNAO1 variants, one(More)