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The TetR Family of Transcriptional Repressors
TLDR
A general profile for the proteins of the TetR family of repressors is developed, made up of 47 amino acid residues that correspond to the helix-turn-helix DNA binding motif and adjacent regions in the three- dimensional structures of TetR, QacR, CprB, and EthR, four family members for which the function and three-dimensional structure are known.
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Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms.
TLDR
Nanowires produced by the oxygenic phototrophic cyanobacterium Synechocystis PCC6803 and the thermophilic, fermentative bacterium Pelotomaculum thermopropionicum reveal that electrically conductive appendages are not exclusive to dissimilatory metal-reducing bacteria and may, in fact, represent a common bacterial strategy for efficient electron transfer and energy distribution.
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Methanogenesis facilitated by electric syntrophy via (semi)conductive iron-oxide minerals.
TLDR
It is suggested that Geobacter grew under syntrophic association with methanogens, and IET could occur via electric currents through (semi)conductive iron-oxide minerals (termed 'electric syntrophy') given the ubiquity of conductive minerals in nature.
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Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented sea water.
We found that bacteria closely related to Alcanivorax became a dominant bacterial population in petroleum-contaminated sea water when nitrogen and phosphorus nutrients were supplied in adequate…
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Sulfuricurvum kujiense gen. nov., sp. nov., a facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium isolated from an underground crude-oil storage cavity.
TLDR
On the basis of physiological and phylogenetic data, strain YK-1(T) should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp.
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Microbial interspecies electron transfer via electric currents through conductive minerals
TLDR
This investigation revealed that electrically conductive magnetite nanoparticles facilitated IET from Geobacter sulfurreducens to Thiobacillus denitrificans, accomplishing acetate oxidation coupled to nitrate reduction, suggesting that microbes use conductive mineral particles as conduits of electrons, resulting in efficient IET and cooperative catabolism.
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RNA-Based Stable Isotope Probing and Isolation of Anaerobic Benzene-Degrading Bacteria from Gasoline-Contaminated Groundwater
TLDR
Results indicate that the Azoarcus population was involved in benzene degradation in the gasoline-contaminated groundwater under denitrifying conditions and suggest that RNA-based SIP identification coupled to phylogenetic screening of nonselective isolates facilitates the isolation of enrichment/isolation-resistant microorganisms with a specific function.
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Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes
TLDR
An operon-trap gfp-expression vector available for shotgun cloning is constructed that allows for the selection of positive clones in liquid cultures by fluorescence-activated cell sorting and the utility of SIGEX was demonstrated by the cloning of aromatic hydrocarbon–induced genes from a groundwater metagenome library and subsequent genome-informatics analysis.
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Plant/microbe cooperation for electricity generation in a rice paddy field
TLDR
The results suggest that the paddy-field electricity-generation system was an ecological solar cell in which the plant photosynthesis was coupled to the microbial conversion of organics to electricity.
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Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater.
TLDR
A Gram-negative, mesophilic bacterial strain, designated 1-1B(T), which degrades polycyclic aromatic hydrocarbons, was isolated from petroleum-contaminated seawater during a bioremediation experiment and represents a novel species within the genus Thalassospira, for which the name ThalASSospira tepidiphila sp.
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