Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
The Homeoprotein Nanog Is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.
Induction of pluripotent stem cells from fibroblast cultures
Detailed methods and tips for the generation of induced pluripotent stem cells, named iPS cells, can be differentiated into three germ layers and committed to chimeric mice are described.
Suppression of induced pluripotent stem cell generation by the p53–p21 pathway
Functional analyses of 34 p53-regulated genes demonstrate that the p53–p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation in mouse and human fibroblasts.
Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.
Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells
Genetic lineage tracings suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required.
Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells
The new work provides an encouraging step toward using motor neurons generated from iPSCs derived from ALS patients to learn more about what triggers the death of motor neurons in this disease and to identify new candidate drugs that may be able to slow or reverse the devastating loss ofMotor neurons.