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Reevaluation and Reduction of a PCR Bias Caused by Reannealing of Templates
ABSTRACT We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause theExpand
First record of the plant bug subfamily Psallopinae (Heteroptera: Miridae) from Thailand, with descriptions of new species and immature forms
The subfamily Psallopinae from continental Southeast Asia is recorded for the first time, and two undescribed species of Psallops and P. fulvioides are described as new to science. Expand
Japanese species of the genus Amphiareus Distant (Heteroptera: Anthocoridae), with descriptions of two new species
Japanese species of the genus Amphiareus Distant of the lyctocorine flower bug are revised and two new species are described and illustrated. Expand
Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids
This work applied the PAI-PCR method to isolate glycosyl hydrolase genes from DNAs extracted from vermiform appendixes of horses and termite guts and amplified and cloned successfully the flanking sequences of the target genes, whereas standard IPCR resulted in no amplification. Expand
Molecular phylogeny and evolutionary habitat transition of the flower bugs (Heteroptera: Anthocoridae).
Reconstruction of the ancestral habitat patterns of the Anthocoridae suggests that dead plants may have served as an important habitat for the common ancestor of anthocorids. Expand
Optimization of a rapid and sensitive identification system for Salmonella enteritidis by capillary electrophoresis with laser-induced fluorescence.
A system coupling capillary electrophoresis with a laser-induced fluorescence (LIF) system for the identification of Salmonella enteritidis was developed and successfully detected as few as three cells per injection from a pure culture of S.Enteritidis. Expand
Direct cloning and expression of putative esterase genes from environmental DNA
This study illustrates that pre-amplified inverse PCR, which is one of the sequence-based approach, is potentially applicable to the isolation of diverse genes from environmental DNA. Expand
Pleistocene sea level fluctuation and host plant habitat requirement influenced the historical phylogeography of the invasive species Amphiareus obscuriceps (Hemiptera: Anthocoridae) in its native
The results suggest that ecological properties (habitat requirement and dispersal ability), together with sea level fluctuation during the Pleistocene across the ECS, have shaped the genetic structure and demographic history of A. obscuriceps. Expand
The genus Physopleurella reuter (Hemiptera: Heteroptera Anthocoridae) from Southeastern Asia
The flower bug genus Physopleurella Reuter 1884 of southeastern Asia is revised, including three new species, including two new species recorded from southeastern Asia for the first time. Expand