Kazutaka Arisue

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We report a method for assaying magnesium in serum and urine involving only one enzyme, isocitrate dehydrogenase (NADP+)(EC 1.1.1.42), which requires magnesium ion for activity. The enzymatic reduction of NADP+ by isocitrate increases in rate linearly up to at least 20 mmol/L magnesium in the presence of appropriate concentrations of the two metal-chelating(More)
The circadian rhythm of plasma corticosteroid (CS) levels in adult female rats was studied chronologically under the following conditions: normal light-dark (LD), inverted light-dark (DL), constant dark (DD) and constant light (LL). Animals were accustomed to LD condition for 7 days before exposure to each abnormal lighting regimen. Normal circadian rhythm(More)
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