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Thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. Among these strains, eight showed significant (74 to 99%) coaggregation with Acinetobacter johnsonii S35 while only four strains coaggregated, to a lesser(More)
The extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of Acinetobacter johnsonii S35 with two other strains of non-flocculating sludge bacteria (Microbacterium esteraromaticum S38 and M. esteraromaticum S51). At first, the effect of electrolyte(More)
The coaggregation traits of two pairs of sewage sludge bacteria were tested and characterized. Oligotropha carboxidovorans S23 coaggregated with two strains of the genus Acinetobacter viz. Acinetobacter junii S33 (56%) and Acinetobacter johnsonii S35 (99%). Coaggregates of O. carboxidovorans S23 and A. junii S33 were small (20-40 microm), weak and(More)
Two strains of non-flocculating sewage sludge bacteria (Xanthomonassp. S53 and Microbacterium esteraromaticum S51) showed 91% and 77% co-aggregation, respectively, with Acinetobacter johnsonii S35 using a spectrophometric assay. The co-aggregates in case of Xanthomonas sp. S53 and A. johnsonii S35 were above 100 μm and stable against EDTA (2 mM) and a(More)
The ability of non-flocculating Acinetobacter spp. isolates (A. johnsonii S35 and A. junii S33) to coaggregate with other sludge-constituting bacteria obtained from a sewage sludge sample was assessed by a spectrophotometric assay. A. johnsonii S35 showed significant coaggregation (aggregation index (AI)=70 to 99%) with 7 strains, Bacillus cereus,(More)
The effect of anionic (sodium dodecyl sulphate or SDS) and cationic (cetyltrimethylammonium bromide or CTAB) surfactants on the stability of binary bacterial coaggregates comprising Acinetobacter johnsonii S35 and Oligotropha carboxidovorans S23 (both sewage sludge isolates) was studied and compared with that on the complex sewage sludge flocs. Both SDS and(More)
The coaggregation behavior of Acinetobacter johnsonii S35 isolate with sewage bacteria was assessed by a spectrophotometric assay using different samples from a municipal wastewater treatment plant and a community plant. A. johnsonii S35 coaggregated well with other free bacteria and microflocs at the mixing ratios of 0.2:1-0.6:1 of A. johnsonii S35 and(More)
In an effort to develop an aerated filter with high nitrification capabilities, we formulated a new filter with 2 layers: the upper layer is filled with a plastic medium, and the lower with anthracite. We tested the filter in a pilot-scale apparatus on secondary-treated sewage, and in a small-scale apparatus on secondary-treated sewage and on artificial(More)
Fusarium phosphodiesterase-phosphomonesterase was purified 1,630-fold with 19% yield from dried powder of the culture medium by a modified method consisting of seven steps. The purified preparation was shown to be devoid of inactive protein by disc electrophoresis. The preparation was homogeneous with respect to size as demonstrated by gel filtration,(More)
Acinetobacter johnsonii S35 has an ability to coaggregate with Oligotropha carboxidovorans S23 when grown with favorable carbon substrate and nutrient-rich growth media. However, nutrient-deficient situations or poorly accessible carbon substrates in waste streams could trigger changes in the cell surface properties and thus modify the coaggregation(More)