Kazunobu Sasaki

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The development and cytoarchitectures of liver hematopoiesis in the mouse from 10 to 19 days of gestation were examined by light and electron microscopy. In fetal liver hematopoiesis, four stages were identified: Stage I, the onset of hematopoiesis at 10 days; Stage II, expansion of the volume of the hematopoietic compartment at 11 and 12 days; Stage III,(More)
Fetal liver hematopoiesis in mice disappears approximately 2 weeks after birth; however, under experimental acute anemia extramedullary hematopoiesis occurs in the livers of adult mice. The hematopoietic foci in the liver during extramedullary hematopoiesis contain erythroblasts and macrophages. In this study, the extent of involvement of macrophages in the(More)
This study used 100-mum thick paraffin sections stained by the ER-HR3 antibody to examine the three-dimensional surface morphology of the central macrophages of erythroblastic islets in the splenic red pulp of aged and pregnant mice. The ER-HR3-positive cells were the macrophages located at the center of the erythroblastic islets, and the number per unit of(More)
The duct epithelial cells of rabbit submandibular glands expressed keratin 8, keratin 14, and actin in the supranuclear region, and these cytoskeletal proteins formed ring structures, approximately 1–2.5 µm in diameter. Ultrastructurally, these ring structures were observed as a 'Golgi-associated filament network' surrounding Golgi apparatuses. Double(More)
Karyometric studies were made of lymphocytes for the cortex and medulla of the thymus of mice at various ages from birth to adulthood. From the results obtained it was evident that in early neonate mice, medullary small lymphocytes were characterized by having larger nuclei than cortical small lymphocytes. On the basis of cytological features, such(More)
Using 100-microm-thick paraffin sections stained by F4/80 antibody, the three-dimensional surface morphology of macrophages in fetal and adult livers was examined by conventional light microscope equipped with a computer-controlled z-axis stepping motor. Hematopoietic macrophages in fetal livers were located in the center of erythroid cell clusters, forming(More)
In mammals, macrophages are known to play an important role in lens development. Macrophages in the embryonic lens are positive for F4/80 monoclonal antibody, and, from 10.5 days to 12 days of gestation, numerous macrophages were observed in the ectoderm, lens vesicle, lens cavity and surrounding mesenchymal tissue, phagocytosing and removing degenerating(More)
Using lectin and colloidal iron (CI) stainings in combination with neuraminidase digestion, glycoconjugates on the surface of erythropoietic cells of the yolk sac and liver in fetal mice were examined. Fetal hepatic macrophages were capable of distinguishing between phagocytozed and non-phagocytozed erythroid elements as described in our previous study.(More)
Glycoconjugates on the surface of pulmonary epithelial cells were ultrahistochemically examined in the fetal, neonatal and adult rat lung. Lectin and colloidal iron staining procedures were performed in combination with digestion using carbohydrate-degrading enzymes or methylation. The glycoconjugate composition of columnar cells at 16 days gestation was(More)
 To clarify the origin of the central cells in hepatic erythroblastic islands, glycoconjugates on the surface of cellular constituents in fetal mice liver were ultrahistochemically examined using lectin staining. At 11 days of gestation, the cells derived from mesenchyme in fetal liver, including sinusoidal macrophages, endothelial cells, and erythropoietic(More)