Kazumasa Matsumoto

Learn More
A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from(More)
An analytical system based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) was established for the precise evaluation of human intestinal microbiota. Group- and species-specific primer sets for Clostridium perfringens, Lactobacillus spp. (six subgroups and three species), Enterococcus spp., and Staphylococcus spp. targeting 16S rRNA gene(More)
For the detection and identification of predominant bacteria in human feces, 16S rRNA-gene-targeted group-specific primers for the Bacteroides fragilis group, Bifidobacterium, the Clostridium coccoides group, and Prevotella were designed and evaluated. The specificity of these primers was confirmed by using DNA extracted from 90 species that are commonly(More)
OBJECTIVES To investigate the association between the expression of nestin, a class VI intermediate filament protein, and pathologic features or survival in patients with urothelial carcinoma of the bladder (UCB). METHODS Nestin expression in tumor cells was immunohistochemically studied in 93 patients with UCB who underwent radical cystectomy with pelvic(More)
—The effective permittivity of the one-dimensional periodic structure is examined using the reflectance and trans-missivity obtained by the FDTD method. By using the reflectance and transmissivity by the FDTD method, the effective permittivity of the dielectric slab, which has the same reflectance and transmissivity with that of periodic structure, is(More)
We report a case of neurofibromatosis type 1 (NF1), also known as von Recklinghausen's disease, with a primary neurofibroma of the penis that was treated surgically. A 38-year-old man presented with severe pain from a penile mass localized in the left aspect of the proximal portion of the penile shaft. The mass was excised, and there was no adhesion between(More)
BACKGROUND Optical coherence tomography (OCT) has become a promising diagnostic tool in many medical fields. In particular, noninvasive real-time optical biopsy of internal organs is one of the most attractive applications of OCT, enabling in situ diagnosis of carcinoma at an early stage. We used an ultra-high speed OCT system for real-time(More)
  • 1