Kazuma Fujimura

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Most recombinant human bone morphogenetic protein (rhBMP) is currently obtained from Chinese hamster ovary (CHO) cells. If rhBMP with more activity could be derived from Escherichia coli (E. coli), a large quantity of rhBMP could be produced at low cost. The bone-inducing ability of an E. coli -derived rhBMP-2 (ErhBMP-2) variant with an N-terminal sequence(More)
Clinically, it would be more convenient to use recombinant than purified preparations of bone morphogenetic protein (BMP). Recently, recombinant human BMP (rhBMP) has attracted the attention of many investigators, but it has not been fully characterized. We examined the bone-inducing activity of rhBMP-2 and compared it with that of purified BMP derived from(More)
AIMS Mesenchymal stem cells are an interesting source of material for regenerative medicine. The present study aimed at characterizing the phenotype and differentiation potential of adherent synovial fluid-derived cells from temporomandibular joint (TMJ) disorder patients. MAIN METHODS Synovial fluid collection takes place during TMJ cavity irrigation(More)
To compare the osteoinductive activity of recombinant human bone morphogenetic protein-2 (rhBMP-2) at various sites in rats, 5 microg of rhBMP-2 were implanted into various sites, using atelopeptide type-I collagen (CL) as a carrier (BMP groups). CL implantation was used as a control. Forty Wistar rats were divided into intramuscular, intermuscular,(More)
OBJECTIVE To measure the levels of various cytokines, cytokine receptors, and cytokine antagonists in the synovial fluid of patients with temporomandibular disorders (TMD) and to determine the correlations among these expression levels. STUDY DESIGN Synovial fluid was obtained from 55 patients with TMD and from 5 asymptomatic healthy volunteers as(More)
Atelopeptide type I collagen derived from fresh porcine skin was evaluated as a carrier for ectopic osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2) in rats. Four treatment groups (N = 5) were examined: a control group in which only atelopeptide type I collagen was implanted, and groups II, III and IV in which atelopeptide type I(More)
The osteoinduction capability of recombinant human bone morphogenetic protein-2 (rhBMP-2) in the muscle and in the subcutaneous tissue in Wistar rats (n = 20) was evaluated, using atelopeptide type-I collagen as a carrier. The alkaline phosphatase (ALP) activity and calcium (Ca) content were quantitatively analyzed 1, 3, 7 and 21 days after the implantation(More)
For skeletal reconstruction, a vascularised bone graft is both reliable and useful, but some sacrifice usually occurs at the skeletal donor site. In the search for our alternative, we investigated ectopic osteoinduction by bone morphogenetic protein in a rat muscle flap, to prefabricate a muscle flap that included bone. Right latissimus dorsi muscle flaps(More)
Into a calf muscle pouch in Wistar rats, 50 micrograms purified bone morphogenetic protein (pBMP) or 50 micrograms recombinant human bone morphogenetic protein-2 (rhBMP-2) was implanted using atelopeptide type I collagen solution (CL) as a carrier. Three weeks later bone and bone marrow were induced in both groups. These induced bone and bone marrow were(More)
PURPOSE The aim of this study was to investigate the ideal volume of perfusate for arthrocentesis of the temporomandibular joint (TMJ) disorders (TMDs). PATIENTS AND METHODS We evaluated 17 joints in 17 patients with TMD in this study. Arthrocentesis of the TMJ was done by perfusion of 400 mL of Ringer's solution. The first 5 mL of perfusate was(More)