Kazuki Matsubara

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Two paradigm shifts in DNA sequencing technologies-from bulk to single molecules and from optical to electrical detection-are expected to realize label-free, low-cost DNA sequencing that does not require PCR amplification. It will lead to development of high-throughput third-generation sequencing technologies for personalized medicine. Although nanopore(More)
We report label-free electrical detections of chemically modified nucleobases in a DNA using a nucleotide-sized electrode gap. We found that methyl substitution contributes to increase the tunneling conductance of deoxycytidines, which was attributed to a shift of the highest occupied molecular orbital level closer to the electrode Fermi level by(More)
We developed a simple fabrication procedure for a fabrication of nanostructures with electron-beam (EB) lithography. We found that, an electron-beam irradiation with a density of over 50 mC/cm have high-resistance against dry-etching, resulting preserve in a clear-pattern of fluidics and through-hole pore for the EB-lithographed image. This electron-beam(More)
Structures of biological surfaces pressed on to a pure liquid gallium surface were successfully traced on to the gallium surface by quick-freezing below the melting point (28.78 degrees C) in air or water for replication in scanning electron microscopy. Gallium's high surface tension (approximately 700 mN m(-1) at 30 degrees C) deteriorates the spatial(More)
We investigated a conductance-time e profiles DNA/RNA oligomers by mechanically controllable nano-break junction (Nano-MCBJ). Based on the difference in the conductance-time profiles, we sequentially identified the base-type in the oligonucleotide just passing through the sensing electrode, resulting in the determination of partial sequence. From the read(More)
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