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Protein glycosylation is one of the most common post-translational modifications in eukaryotes and affects various aspects of protein structure and function. To facilitate studies of protein glycosylation, we paired glycosylation site-specific stable isotope tagging of lectin affinity-captured N-linked glycopeptides with mass spectrometry and determined(More)
In gene expression analysis, target genes are amplified by reverse transcription polymerase chain reaction (RT-PCR) with messenger RNA (mRNA). We previously reported a microfluidic device for mRNA isolation by the combination of a micromill and a microshredder. In this study, we report an RT-PCR micromodule that can operate simultaneously with above device.(More)
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